What application is this antibody validated for?

This Mouse Anti-TNFSF15 Recombinant Antibody is validated for Enzyme-Linked Immunosorbent Assay (ELISA). It is designed to recognize Human TNFSF15 (TL1A).

Is this a purified antibody?

Yes, this antibody is purified by affinity chromatography. It is expressed in mammalian cells with chemically defined culture media, ensuring high purity (>95% by SDS-PAGE).

Mouse Anti-TNFSF15 Recombinant Antibody (HPAB-1443-FY)

CAT#: HPAB-1443-FY

This product is a recombinant mouse antibody that recognizes TNFSF15. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line RT4

Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & nuclear membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46522/1383_A7_2_selected.jpg

Normal Tissue Figure 2 IHC staining of human esophagus

Immunohistochemical staining of human esophagus shows strong membranous positivity in squamous epithelial cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_B_4_1_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_B_9_5.jpg

Normal Tissue Figure 4 Colon

Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_A_8_3.jpg

Normal Tissue Figure 5 Kidney

Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_A_8_5.jpg

Normal Tissue Figure 6 Testis

Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_A_6_6.jpg

Normal Tissue Figure 7 Skin

Epidermal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_B_5_8.jpg

Normal Tissue Figure 8 Lymph node

Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_A_9_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enhanced.

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000181634-TNFSF15

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Specifications

Host Species
Mouse

Type
Mouse IgG

Specificity
Human TNFSF15

Species Reactivity
Human

Applications
ELISA

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
TNF Superfamily Member 15; Vascular Endothelial Cell Growth Inhibitor; TNF Ligand-Related Molecule 1; Tumor Necrosis Factor (Ligand) Superfamily; Member 15; Vascular Endothelial Growth Inhibitor-192A; TNF Superfamily Ligand TL1A; VEGI; TL1

Gene ID
9966

UniProt ID
O95150

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Breast cancer biomarkers at key points during disease progression

Sensitive Cytokine Detection

We developed an ELISA using this Recombinant Mouse Anti-TNFSF15 antibody to measure cytokine release from endothelial cells. The antibody showed high sensitivity and specificity for the target. It helped us quantify the expression levels of TL1A under inflammatory conditions accurately.

Consistent Recombinant Quality

Having a recombinant source for this antibody is a major advantage. We have used it over several months for different experiments, and the performance has remained stable. It reliably detects TNFSF15, making it a dependable reagent for our vascular biology research.

Q&As

What application is this antibody validated for?

A: This Mouse Anti-TNFSF15 Recombinant Antibody is validated for Enzyme-Linked Immunosorbent Assay (ELISA). It is designed to recognize Human TNFSF15 (TL1A).
Is this a purified antibody?

A: Yes, this antibody is purified by affinity chromatography. It is expressed in mammalian cells with chemically defined culture media, ensuring high purity (>95% by SDS-PAGE).

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-1443-FY.pdf

MSDS

MSDS - HPAB-1443-FY.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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