AbPlus™ Anti-TNNI3 Magnetic Beads (1542.14A2)
CAT#: VS-0424-XY260
The AbPlus Anti-TNNI3 Magnetic Beads (1542.14A2) is an innovative affinity resin which is bound with anti-TNNI3 specific antibody (1542.14A2). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of TNNI3 protein under native and denaturing conditions.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1153_D2_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1220_C7_6_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1118_D2_2_blue_red_green.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_A_6_6.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_B_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HEK93, HMC-1, K-562, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129991-TNNI3
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-TNNI3 specific antibody (1542.14A2)
- Target
- TNNI3
- Immunogen
- Recombinant human cardiac troponin I (cTnI) antigen
- Target Species
- Human
- Antibody Clone
- 1542.14A2
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
- Alternative Names
- TNNI3; troponin I type 3 (cardiac); CMH7; RCM1; cTnI; CMD2A; TNNC1; MGC116817; troponin I, cardiac; Troponin I, cardiac muscle; Cardiac troponin I; familial hypertrophic cardiomyopathy 7
- Gene ID
- 7137
- UniProt ID
- P19429
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Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1096 | Human Anti-TNNI3 Recombinant Antibody (clone 1542.14A2); scFv Fragment | ELISA, WB, IHC, IP | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1099 | Human Anti-TNNI3 Recombinant Antibody (clone 1542.14A2); scFv Fragment, HRP | ELISA, WB, IHC, IP | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-733 | Chicken Anti-TNNI3 Recombinant Antibody (clone mAb180) | WB, IHC, IF, FuncS | Chicken IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0022MZ | Recombinant Mouse Anti-Human TNNI3 Antibody (clone C159M) | IA, IP, IHC, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2209CQ | Mouse Anti-TNNI3 Recombinant Antibody (NEUT-2209CQ) | ELISA, Inhib, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2210CQ | Mouse Anti-TNNI3 Recombinant Antibody (VMC208) | ELISA, Inhib, WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2211CQ | Mouse Anti-TNNI3 Recombinant Antibody (clone 4C2) | ELISA, Inhib, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3627 | Rabbit Anti-TNNI3 Recombinant Antibody (clone DS3627AB) | ICC, IF, IHC-Fr, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0154-CN | Rabbit Anti-TNNI3 Recombinant Antibody (clone MG4) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0154-CN-S(P) | Rabbit Anti-TNNI3 Recombinant Antibody (clone MG4); scFv Fragment | ELISA | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0154-CN-F(E) | Rabbit Anti-TNNI3 Recombinant Antibody (clone MG4); Fab Fragment | ELISA | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0232-CN | Rabbit Anti-TNNI3 Recombinant Antibody (clone JF0550) | WB, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0942-FY | Mouse Anti-TNNI3 Recombinant Antibody (HPAB-0942-FY) | FuncS, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0942-FY-F(E) | Mouse Anti-TNNI3 Recombinant Antibody Fab Fragment (HPAB-0942-FY-F(E)) | FuncS, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0942-FY-S(P) | Mouse Anti-TNNI3 Recombinant Antibody scFv Fragment (HPAB-0942-FY-S(P)) | FuncS, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0497-YJ-S(P) | Mouse Anti-TNNI3 Recombinant Antibody (clone CBT62YJ); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0497-YJ-F(E) | Mouse Anti-TNNI3 Recombinant Antibody (clone CBT62YJ); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2324 | Anti-TNNI3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7407 | Anti-Mouse TNNI3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7406 | Anti-Human TNNI3 Immunohistochemistry Kit | IHC |
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