Rabbit Anti-TNNI3 Recombinant Antibody (clone JF0550)
CAT#: MRO-0232-CN
This antibody is a rabbit monoclonal antibody that is capable of binding to TNNI3.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1153_D2_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1220_C7_6_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1118_D2_2_blue_red_green.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_A_6_6.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_B_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HEK93, HMC-1, K-562, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129991-TNNI3
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Specifications
- Immunogen
- Human Cardiac Troponin I, aa 1-150
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TNNI3
- Species Reactivity
- Human, Mouse
- Clone
- JF0550
- Applications
- WB, IF, IHC, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 1*TBS, pH 7.4, 1% BSA, 40% Glycerol
- Preservative
- 0.05% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
REVIEWS AND Q&AS
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Specific Cardiac Marker
We utilized clone JF0550 for immunohistochemistry on cardiac tissue sections. It specifically stained Cardiac Troponin I (TNNI3), distinguishing it from skeletal isoforms. The rabbit monoclonal antibody provided a clean and strong signal, making it a reliable marker for our cardiomyopathy studies.
Consistent Western Blot
This antibody performed very well in Western blot analysis of cardiac muscle lysates. We detected TNNI3 in both human and mouse samples. The product is high purity (>95%) and the liquid formulation is convenient. It is a staple reagent in our lab for muscle protein analysis.
Q&As
-
What is the immunogen used for this TNNI3 antibody?
A: The antibody (clone JF0550) was produced using Human Cardiac Troponin I (aa 1-150) as the immunogen. It is a Rabbit monoclonal antibody that reacts with Human and Mouse TNNI3.
-
What applications is this antibody suitable for?
A: This antibody is suitable for Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), and Immunoprecipitation (IP). It is Protein A purified and shows high specificity for the cardiac isoform.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "TNNI3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1096 | Human Anti-TNNI3 Recombinant Antibody (clone 1542.14A2); scFv Fragment | ELISA, WB, IHC, IP | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1099 | Human Anti-TNNI3 Recombinant Antibody (clone 1542.14A2); scFv Fragment, HRP | ELISA, WB, IHC, IP | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-733 | Chicken Anti-TNNI3 Recombinant Antibody (clone mAb180) | WB, IHC, IF, FuncS | Chicken IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0022MZ | Recombinant Mouse Anti-Human TNNI3 Antibody (clone C159M) | IA, IP, IHC, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2209CQ | Mouse Anti-TNNI3 Recombinant Antibody (NEUT-2209CQ) | ELISA, Inhib, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2210CQ | Mouse Anti-TNNI3 Recombinant Antibody (VMC208) | ELISA, Inhib, WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2211CQ | Mouse Anti-TNNI3 Recombinant Antibody (clone 4C2) | ELISA, Inhib, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3627 | Rabbit Anti-TNNI3 Recombinant Antibody (clone DS3627AB) | ICC, IF, IHC-Fr, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0154-CN | Rabbit Anti-TNNI3 Recombinant Antibody (clone MG4) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0154-CN-S(P) | Rabbit Anti-TNNI3 Recombinant Antibody (clone MG4); scFv Fragment | ELISA | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0154-CN-F(E) | Rabbit Anti-TNNI3 Recombinant Antibody (clone MG4); Fab Fragment | ELISA | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0942-FY | Mouse Anti-TNNI3 Recombinant Antibody (HPAB-0942-FY) | FuncS, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0942-FY-F(E) | Mouse Anti-TNNI3 Recombinant Antibody Fab Fragment (HPAB-0942-FY-F(E)) | FuncS, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0942-FY-S(P) | Mouse Anti-TNNI3 Recombinant Antibody scFv Fragment (HPAB-0942-FY-S(P)) | FuncS, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0497-YJ | Mouse Anti-TNNI3 Recombinant Antibody (clone CBT62YJ) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0497-YJ-S(P) | Mouse Anti-TNNI3 Recombinant Antibody (clone CBT62YJ); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0497-YJ-F(E) | Mouse Anti-TNNI3 Recombinant Antibody (clone CBT62YJ); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY260 | AbPlus™ Anti-TNNI3 Magnetic Beads (1542.14A2) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2324 | Anti-TNNI3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7407 | Anti-Mouse TNNI3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7406 | Anti-Human TNNI3 Immunohistochemistry Kit | IHC |
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