Mouse Anti-TNNI3 Recombinant Antibody (ZG-160R)
CAT#: ZG-160R
This product is a mouse antibody that recognizes TNNI3. It can be used in a wide variety of applications, such as: WB, ELISA, IHC.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-TNNI3 Antibody (ZG-160R) in WB
Western Blot analysis using Troponin I-C monoclonal antibody against truncated Troponin I-C recombinant protein.
IHC
Figure 2 Mouse Anti-TNNI3 Antibody (ZG-160R) in IHC
Immunohistochemistry analysis of paraffin-embedded human normal cardiac muscle tissue, showing cytoplasmic localization with DAB staining using Troponin I-C monoclonal antibody.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1153_D2_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1220_C7_6_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1118_D2_2_blue_red_green.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_A_6_6.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_B_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HEK93, HMC-1, K-562, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129991-TNNI3
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Specifications
- Immunogen
- Purified recombinant fragment of Troponin I-C expressed in E. coli
- Host Species
- Mouse
- Type
- Mouse IgG
- Antibody Isotype
- IgG
- Species Reactivity
- Human
- Applications
- WB, ELISA, IHC
Product Property
- Purity
- >95%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:2000, IHC: 1:200-1:1000, ELISA: 1:10000
Target
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Specific Cardiac Marker
We used this mouse antibody to detect TNNI3 in cardiac tissue samples via IHC. The staining was specific to the cardiac muscle as expected, with no background in skeletal muscle controls. It is a reliable antibody for studying cardiac troponin I expression.
Effective in Western Blot
The antibody performed very well in Western Blotting, detecting the TNNI3 protein at the correct size. We used dilutions within the recommended range of 1:500-1:2000, and the signal was strong. The high purity of the recombinant fragment is evident in the clean results.
Q&As
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What tissue does TNNI3 expression indicate?
A: TNNI3 (Troponin I, cardiac type) is exclusively expressed in cardiac muscle tissues. This antibody recognizes TNNI3 and is useful for detecting this specific subunit of the troponin complex.
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What are the recommended dilutions for Western Blot?
A: For Western Blot (WB), we recommend a starting dilution range of 1:500 to 1:2000. However, optimal working dilutions should always be determined experimentally by the end user.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| FAMAB-0154-CN-F(E) | Rabbit Anti-TNNI3 Recombinant Antibody (clone MG4); Fab Fragment | ELISA | Rabbit Fab |
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| HPAB-0497-YJ-F(E) | Mouse Anti-TNNI3 Recombinant Antibody (clone CBT62YJ); Fab Fragment | ELISA | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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