Mouse Anti-TNNT2 Recombinant Antibody (clone 4B1D5)
CAT#: VS7-0425-WR1032
This product is a mouse IgG1 antibody that binds to human TNNT2. The antibody 4B1D5 recognizes specific amino acid domain of human TNNT2 (AA: 1-295). It can be used for IHC and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm, nucleoli & microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17888/1417_H7_4_selected.jpg
Normal Tissue
Figure 2 High expression in heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15371/34258_B_5_6.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (RH-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118194-TNNT2
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Specifications
- Immunogen
- Recombinant human TNNT2 (AA: 1-295) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TNNT2
- Species Reactivity
- Human
- Clone
- 4B1D5
- Molecule Class
- Tropomyosin-Binding Subunit Of The Troponin Complex
- Applications
- IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Cardiomyopathy, familial hypertrophic, 2 (CMH2); Cardiomyopathy, dilated, 1D (CMD1D); Cardiomyopathy, familial restrictive 3 (RCM3)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- Troponin T2, Cardiac Type; Cardiomyopathy, Dilated 1D (Autosomal Dominant); Cardiomyopathy, Hypertrophic 2; Troponin T Type 2 (Cardiac); TnTC; CTnT; Truncated Cardiac Troponin T; Troponin T, Cardiac Muscle
- Gene ID
- 7139
- UniProt ID
- P45379
- Long Name
- TNNT2 Gene(Protein Coding) Troponin T2, Cardiac Type
- Cellular Localization
- cardiac myofibril; cytosol; sarcomere; striated muscle thin filament
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_000355.2
- Function
- Muscle protein
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "TNNT2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0227MZ | Recombinant Mouse Anti-Troponin T-Cardiac 1-60 Region Antibody (clone 10H7) | ELISA | Mouse antibody |
| MRO-0233-CN | Mouse Anti-TNNT2 Recombinant Antibody (clone 3-G6) | WB, IHC, IF | Mouse IgG2b |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2212CQ | Mouse Anti-TNNT2 Recombinant Antibody (clone 1F11) | ELISA, Inhib, WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3628 | Rabbit Anti-TNNT2 Recombinant Antibody (clone DS3628AB) | IHC-P, IP, WB | Rabbit IgG |
| MOR-4516 | Rabbit Anti-TNNT2 Recombinant Antibody (clone TH24DS) | ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7408 | Anti-Mouse TNNT2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7409 | Anti-Rat TNNT2 Immunohistochemistry Kit | IHC |
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