Mouse Anti-TOP1 Recombinant Antibody (clone 1291CT875.142.166) (CAT#: VS3-QX1142)
This product is a mouse antibody that recognizes TOP1. The antibody can be used in applications: WB, FC.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/154_H7_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/154_H7_2_selected.jpg
Figure 2 IHC staining of human appendix
(Immunohistochemical staining of human appendix shows general nuclear positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_3_2_selected.jpg
(Immunohistochemical staining of human appendix shows general nuclear positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_3_2_selected.jpg
Figure 3 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9058/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9058/ihc_selected.jpg
Figure 4 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/198_H7_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/198_H7_1_red_green.jpg
Figure 5 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/152_H7_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/152_H7_1_red_green.jpg
Figure 6 Cerebral cortex
(Glial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_B_9_5.jpg
(Glial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_B_9_5.jpg
Figure 7 Colon
(Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_7_3.jpg
(Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_7_3.jpg
Figure 8 Kidney
(Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_8_5.jpg
(Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_8_5.jpg
Figure 9 Urinary bladder
(Urothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_5_5.jpg
(Urothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_5_5.jpg
Figure 10 Testis
(Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_4_6.jpg
(Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_4_6.jpg
Figure 11 Lymph node
(Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_9_8.jpg
(Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_9_8.jpg
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000198900-TOP1
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000198900-TOP1
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198900-TOP1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198900-TOP1
Specifications
- Immunogen
- A recombinant protein from the N-terminal region of human TOP1.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- TOP1 (N-term)
- Species Reactivity
- Human
- Clone
- 1291CT875.142.166
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1:1000; FC: 1:25
Target
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibodies
IgG Antibodies
Fab Antibodies
ScFv Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3638 | Rabbit Anti-TOP1 Recombinant Antibody (clone DS3638AB) | FC, ICC, IF, IHC-P, WB | Rabbit IgG |
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