Mouse Anti-TOP2A Recombinant Antibody (clone 6C12G12) (CAT#: VS7-0425-WR1033)
This product is a mouse IgG2b antibody that binds to human TOP2A. The antibody 6C12G12 recognizes specific amino acid domain of human TOP2A (AA: 1100-1530). It can be used for WB, ICC, and FC assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6458/73_E8_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6458/73_E8_2_selected.jpg
Figure 2 Testis
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2448/6156_A_5_6.jpg
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2448/6156_A_5_6.jpg
Figure 3 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131747-TOP2A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131747-TOP2A
Specifications
- Immunogen
- Recombinant human TOP2A (AA: 1100-1530) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human TOP2A
- Species Reactivity
- Human
- Clone
- 6C12G12
- Molecule Class
- Dna Topoisomerase
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:100 - 1:500
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- TOP2A; Topoisomerase (DNA) II Alpha (170kD); DNA Topoisomerase 2-Alpha; TP2A; TOP2; DNA Topoisomerase II, 170 KD; DNA Topoisomerase (ATP-Hydrolyzing); DNA Topoisomerase II, Alpha Isozyme
- Gene ID
- 7153
- UniProt ID
- P11388
- Long Name
- DNA Topoisomerase II Alpha
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001058.2
- Function
- DNA-binding; Isomerase; Topoisomerase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1962z | Mouse Anti-TOP2A Recombinant Antibody (clone 40D5) | WB, FC, IF, IHC | Mouse IgG2b, κ |
| MOB-1812MZ | Recombinant Mouse Anti-Human TOP2A Antibody (clone Lj-T2) | IHC-P | Mouse antibody |
| MRO-1512-CN | Rabbit Anti-TOP2A Recombinant Antibody (clone CBACN-556) | WB, IHC, IP | Rabbit IgG |
| VS3-FY1471 | Recombinant Mouse Anti-TOP2A Antibody (clone 1D2-2H6-7D1) | IHC-P | Mouse IgG2b |
| VS3-FY2905 | Mouse Anti-TOP2A Recombinant Antibody (VS3-FY2905) | WB, ICC, FC, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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