Mouse Anti-TOP2A Recombinant Antibody (clone 6D4B2)
CAT#: VS7-0425-WR1034
This product is a mouse IgG1 antibody that binds to human TOP2A. The antibody 6D4B2 recognizes specific amino acid domain of human TOP2A (AA: 1100-1530). It can be used for WB and IHC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6458/73_E8_2_selected.jpg
Normal Tissue
Figure 2 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2448/6156_A_5_6.jpg
RNA Expression
Figure 3 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131747-TOP2A
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Specifications
- Immunogen
- Recombinant human TOP2A (AA: 1100-1530) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TOP2A
- Species Reactivity
- Human
- Clone
- 6D4B2
- Molecule Class
- Dna Topoisomerase
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- TOP2A; Topoisomerase (DNA) II Alpha (170kD); DNA Topoisomerase 2-Alpha; TP2A; TOP2; DNA Topoisomerase II, 170 KD; DNA Topoisomerase (ATP-Hydrolyzing); DNA Topoisomerase II, Alpha Isozyme
- Gene ID
- 7153
- UniProt ID
- P11388
- Long Name
- DNA Topoisomerase II Alpha
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001058.2
- Function
- DNA-binding; Isomerase; Topoisomerase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "TOP2A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3639 | Rabbit Anti-TOP2A Recombinant Antibody (clone DS3639AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2235-CN | Rabbit Anti-TOP2A Polyclonal Antibody (MRO-2235-CN) | WB, IF, FC | Rabbit IgG |
| MRO-2237-CN | Rabbit Anti-TOP2A Polyclonal Antibody (MRO-2237-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0129C | Mouse Anti-TOP2A Recombinant Antibody (clone ABT-TOPO) | IHC-P, IF, ICC | Mouse IgG |
| ZG-0130C | Mouse Anti-TOP2A Recombinant Antibody (clone PT0247) | IHC-P, IF, ICC | Mouse IgG |
| ZG-0598U | Rabbit Anti-TOP2A Recombinant Antibody (clone 5B10) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF296 | A*02:01/Human Topoisomerase II-alpha-b (FLYDDNQRV) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7427 | Anti-TOP2A Immunohistochemistry Kit | IHC | |
| VS-0525-XY7428 | Anti-Mouse TOP2A Immunohistochemistry Kit | IHC |
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