Mouse Anti-TOP2A Recombinant Antibody (VS3-CJ593)
CAT#: VS3-CJ593
This product is a mouse antibody that recognizes human TOP2A.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6458/73_E8_2_selected.jpg
Normal Tissue
Figure 2 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2448/6156_A_5_6.jpg
RNA Expression
Figure 3 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131747-TOP2A
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Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence within human Topoisomerase 2 alpha
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human TOP2A
- Species Reactivity
- Human
- Applications
- WB, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.2% BSA, PBS.
- Preservative
- 0.01% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:1000), Immunofluorescence (1:100-1:500), Immunohistochemistry (1:100-1:300).
Target
- Alternative Names
- TOP2; TP2A; TOPIIA; TOP2alpha
- Gene ID
- 7153
- UniProt ID
- P11388
- Sequence Similarities
- Belongs to the type II topoisomerase family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.
- Protein Refseq
- NP_001058.2
- Function
- Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612).
May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "TOP2A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3639 | Rabbit Anti-TOP2A Recombinant Antibody (clone DS3639AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1512-CN | Rabbit Anti-TOP2A Recombinant Antibody (clone CBACN-556) | WB, IHC, IP | Rabbit IgG |
| MRO-2235-CN | Rabbit Anti-TOP2A Polyclonal Antibody (MRO-2235-CN) | WB, IF, FC | Rabbit IgG |
| ZG-0129C | Mouse Anti-TOP2A Recombinant Antibody (clone ABT-TOPO) | IHC-P, IF, ICC | Mouse IgG |
| ZG-0130C | Mouse Anti-TOP2A Recombinant Antibody (clone PT0247) | IHC-P, IF, ICC | Mouse IgG |
| VS3-FY2905 | Mouse Anti-TOP2A Recombinant Antibody (VS3-FY2905) | WB, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF296 | A*02:01/Human Topoisomerase II-alpha-b (FLYDDNQRV) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7427 | Anti-TOP2A Immunohistochemistry Kit | IHC | |
| VS-0525-XY7428 | Anti-Mouse TOP2A Immunohistochemistry Kit | IHC |
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