Mouse Anti-TOP2A Recombinant Antibody (VS3-CJ593) (CAT#: VS3-CJ593)
This product is a mouse antibody that recognizes human TOP2A.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6458/73_E8_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6458/73_E8_2_selected.jpg
Figure 2 Testis
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2448/6156_A_5_6.jpg
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2448/6156_A_5_6.jpg
Figure 3 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131747-TOP2A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131747-TOP2A
Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence within human Topoisomerase 2 alpha
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human TOP2A
- Species Reactivity
- Human
- Applications
- WB, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.2% BSA, PBS.
- Preservative
- 0.01% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:1000), Immunofluorescence (1:100-1:500), Immunohistochemistry (1:100-1:300).
Target
- Alternative Names
- TOP2; TP2A; TOPIIA; TOP2alpha
- Gene ID
- 7153
- UniProt ID
- P11388
- Sequence Similarities
- Belongs to the type II topoisomerase family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.
- Protein Refseq
- NP_001058.2
- Function
- Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612).
May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Recommended Products
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1812MZ | Recombinant Mouse Anti-Human TOP2A Antibody (clone Lj-T2) | IHC-P | Mouse antibody |
| VS3-FY1471 | Recombinant Mouse Anti-TOP2A Antibody (clone 1D2-2H6-7D1) | IHC-P | Mouse IgG2b |
| VS3-FY2905 | Mouse Anti-TOP2A Recombinant Antibody (VS3-FY2905) | WB, ICC, FC, ELISA | Mouse IgG |
| VS7-0425-WR1033 | Mouse Anti-TOP2A Recombinant Antibody (clone 6C12G12) | WB, ICC, FC | Mouse IgG2b |
| VS7-0425-WR1034 | Mouse Anti-TOP2A Recombinant Antibody (clone 6D4B2) | WB, IHC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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