Mouse Anti-TOP2A Recombinant Antibody (VS3-CJ593) (CAT#: VS3-CJ593)

• Immunogen
• KLH-conjugated synthetic peptide encompassing a sequence within human Topoisomerase 2 alpha

• Host Species
• Mouse

• Type
• Mouse IgG2b

• Specificity
• Human TOP2A

• Species Reactivity
• Human

• Applications
• WB, IF, IHC

• Conjugate
• Unconjugated

• Purification
• Immunogen affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.2% BSA, PBS.

• Preservative
• 0.01% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipped at 4°C

• Application Notes
• This antibody has been tested for use in Western Blot (1:500-1:1000), Immunofluorescence (1:100-1:500), Immunohistochemistry (1:100-1:300).

• Alternative Names
• TOP2; TP2A; TOPIIA; TOP2alpha

• Gene ID
• 7153

• UniProt ID
• P11388

• Sequence Similarities
• Belongs to the type II topoisomerase family.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.

• Protein Refseq
• NP_001058.2

• Function
• Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612).
  May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).

Secondary Antibody

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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