Rabbit Anti-TOP2A Recombinant Antibody (VS3-CJ594) (CAT#: VS3-CJ594)
This product is a rabbit antibody that recognizes human, mouse, and rat TOP2A.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6458/73_E8_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6458/73_E8_2_selected.jpg
Figure 2 Testis
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2448/6156_A_5_6.jpg
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2448/6156_A_5_6.jpg
Figure 3 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131747-TOP2A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131747-TOP2A
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat TOP2A
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunohistochemistry, Immunoprecipitation.
Target
- Alternative Names
- TOP2; TP2A; TOPIIA; TOP2alpha
- Gene ID
- 7153
- UniProt ID
- P11388
- Sequence Similarities
- Belongs to the type II topoisomerase family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.
- Protein Refseq
- NP_001058.2
- Function
- Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612).
May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "TOP2A"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1962z | Mouse Anti-TOP2A Recombinant Antibody (clone 40D5) | WB, FC, IF, IHC | Mouse IgG2b, κ |
| MOB-1812MZ | Recombinant Mouse Anti-Human TOP2A Antibody (clone Lj-T2) | IHC-P | Mouse antibody |
| ZG-0129C | Mouse Anti-TOP2A Recombinant Antibody (clone ABT-TOPO) | IHC-P, IF/ICC | Mouse IgG |
| ZG-0130C | Mouse Anti-TOP2A Recombinant Antibody (clone PT0247) | IHC-P, IF/ICC | Mouse IgG |
| VS3-FY1471 | Recombinant Mouse Anti-TOP2A Antibody (clone 1D2-2H6-7D1) | IHC-P | Mouse IgG2b |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3639 | Rabbit Anti-TOP2A Recombinant Antibody (clone DS3639AB) | ICC, IHC-P, WB | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF296 | A*02:01/Human Topoisomerase II-alpha-b (FLYDDNQRV) MHC Monomer | MHC Multimer |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-CJ594. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.