Rabbit Anti-TPBG Recombinant Antibody (clone 6E9) (CAT#: ZG-0047U)
The purified Rabbit Anti-TPBG Recombinant Antibody (clone 6E9) displayed high specificity against the TPBG protein of human and mouse.
Specific Inquiry
WB
Figure 1 Rabbit Anti-TPBG Antibody (ZG-0047U) in WB
Positive WB detected in: U-87 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate. All lanes: 5T4 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 50, 80 kDa
IHC
Figure 2 Rabbit Anti-TPBG Antibody (ZG-0047U) in IHC
IHC image of ZG-0047U diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human 5T4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TPBG
- Species Reactivity
- Human, Mouse
- Clone
- 6E9
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Congenital Disorder Of Glycosylation, Type Il and Renal Cell Carcinoma, Nonpapillary
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Trophoblast glycoprotein; 5T4 oncofetal antigen; 5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; M6P1; Wnt-activated inhibitory factor 1; WAIF1; TPBG; 5T4
- Gene ID
- 7162
- UniProt ID
- Q13641
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-118-F(E) | Anti-Human 5T4 Recombinant Antibody Fab Fragment, 5T4 (TAB-118-F(E)) | FuncS, IF, Neut, ELISA, FC, IP, ICC | Fab - G1 - kappa |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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