Rabbit Anti-TPBG Recombinant Antibody (clone 6E9)
CAT#: ZG-0047U
The purified Rabbit Anti-TPBG Recombinant Antibody (clone 6E9) displayed high specificity against the TPBG protein of human and mouse.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-TPBG Antibody (ZG-0047U) in WB
Positive WB detected in: U-87 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate. All lanes: 5T4 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 50, 80 kDa
IHC
Figure 2 Rabbit Anti-TPBG Antibody (ZG-0047U) in IHC
IHC image of ZG-0047U diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/if_selected.jpg
Normal Tissue
Figure 2 Seminal vesicle
High expression in seminal vesicle
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/26240_A_7_6_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/26240_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/26240_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/26240_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/26240_A_8_5.jpg
Normal Tissue
Figure 7 Placenta
Decidual cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/26240_A_2_7.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced.
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000146242-TPBG
❮
❯
Specifications
- Immunogen
- A synthesized peptide derived from human 5T4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TPBG
- Species Reactivity
- Human, Mouse
- Clone
- 6E9
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Congenital Disorder Of Glycosylation, Type Il and Renal Cell Carcinoma, Nonpapillary
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0047U. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "TPBG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-118-F(E) | Anti-Human 5T4 Recombinant Antibody Fab Fragment, 5T4 (TAB-118-F(E)) | FuncS, IF, Neut, ELISA, FC, IP, ICC | Fab - G1 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1796MZ | Recombinant Mouse Anti-Human TPBG Antibody (clone 3D6) | ELISA, WB | Mouse antibody |
| HPAB-0645-WJ | Human Anti-TPBG Recombinant Antibody (HPAB-0645-WJ) | ELISA | Humanized IgG, κ |
| ZG-0606U | Rabbit Anti-TPBG Recombinant Antibody (clone 1D4) | ELISA, IHC | Rabbit IgG |
| VS-0723-WK11 | Human Anti-TPBG Recombinant Antibody (VS-0723-WK11) | ELISA, FC | Human IgG1 |
| VS-0723-WK12 | Human Anti-TPBG Recombinant Antibody (VS-0723-WK12) | ELISA, FC | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3649 | Rabbit Anti-TPBG Recombinant Antibody (clone DS3649AB) | WB, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0088CQ-S(P) | Human Anti-TPBG Recombinant Antibody; scFv Fragment (HPAB-0088CQ-S(P)) | ELISA, FC | Human scFv |
| HPAB-0089CQ-S(P) | Human Anti-TPBG Recombinant Antibody; scFv Fragment (HPAB-0089CQ-S(P)) | ELISA, FC | Human scFv |
| HPAB-0090CQ-S(P) | Human Anti-TPBG Recombinant Antibody; scFv Fragment (HPAB-0090CQ-S(P)) | ELISA, FC | Human scFv |
| HPAB-0091CQ-S(P) | Mouse Anti-TPBG Recombinant Antibody; scFv Fragment (HPAB-0091CQ-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-0645-WJ-S(P) | Human Anti-TPBG Recombinant Antibody; scFv Fragment (HPAB-0645-WJ-S(P)) | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0088CQ-F(E) | Human Anti-TPBG Recombinant Antibody; Fab Fragment (HPAB-0088CQ-F(E)) | ELISA, FC | Human Fab |
| HPAB-0089CQ-F(E) | Human Anti-TPBG Recombinant Antibody; Fab Fragment (HPAB-0089CQ-F(E)) | ELISA, FC | Human Fab |
| HPAB-0090CQ-F(E) | Human Anti-TPBG Recombinant Antibody; Fab Fragment (HPAB-0090CQ-F(E)) | ELISA, FC | Human Fab |
| HPAB-0287-WJ-F(E) | Rabbit Anti-TPBG Recombinant Antibody; Fab Fragment (HPAB-0287-WJ-F(E)) | ELISA, FC | Rabbit Fab |
| HPAB-0288-WJ-F(E) | Human Anti-TPBG Recombinant Antibody; Fab Fragment (HPAB-0288-WJ-F(E)) | ELISA, FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1281 | PE-H-2Db/Human TPBG (ASFRNLTHL) MHC Tetramer | FCM, IHC | |
| MHC-CN1282 | APC-H-2Db/Human TPBG (ASFRNLTHL) MHC Tetramer | FCM, IHC | |
| MHC-CN1283 | FITC-H-2Db/Human TPBG (ASFRNLTHL) MHC Tetramer | FCM, IHC | |
| MHC-YF748 | H-2Db/Human TPBG (ASFRNLTHL) MHC Pentamer | FCM | |
| MHC-YF755 | A*02:01/Human Trophoblast glycoprotein (RLARLALVL) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY114 | Human Anti-TPBG (clone ASN004) scFv-Fc Chimera | ELISA, FC, FuncS | Human IgG1, scFv-Fc |
| VS-0425-FY60 | Human Anti-TPBG (clone H8) scFv-Fc Chimera | FC, ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC638 | Recombinant Anti-TPBG Vesicular Antibody, EV Displayed (VS-0425-YC638) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7454 | Anti-TPBG Immunohistochemistry Kit | IHC | |
| VS-0525-XY7455 | Anti-Mouse TPBG Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, FC, Inhib, FuncS
Application: Neut, ELISA, IF, IP, FuncS, FC
Application: FC
Application: ELISA, FC, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
