Rat Anti-Trem1 Agonistic Antibody (VS-0724-XY57) (CAT#: VS-0724-XY57)
This antibody is a rat agonistic antibody binding with Trem1 (Triggering receptor expressed on myeloid cells 1), which enables scaffold protein binding activity. The antibody specifically reacts with mouse Trem1. It has been detected using Western Blot, Flow cytometry, Agonistic assays, Immunocytochemistry and Mass Cytometry. The recommended dilutions of Western Blot, Flow cytometry and Immunocytochemistry is 1 µg/mL, 0.25 µg/106 cells and 8-25 µg/mL.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53612/1743_C3_18_cr57fe217b31caf_selected.jpg
(Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53612/1743_C3_18_cr57fe217b31caf_selected.jpg
Figure 2 IHC staining of human liver
(Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5563/ihc_selected.jpg
(Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5563/ihc_selected.jpg
Figure 3 IF staining of human cell line SuSa
(Immunofluorescent staining of human cell line SuSa shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53612/1968_F4_2_blue_red_green.jpg
(Immunofluorescent staining of human cell line SuSa shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53612/1968_F4_2_blue_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5563/15822_B_7_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5563/15822_B_7_5.jpg
Figure 5 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5563/15822_A_7_3.jpg
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5563/15822_A_7_3.jpg
Figure 6 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5563/15822_A_9_4.jpg
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5563/15822_A_9_4.jpg
Figure 7 Urinary bladder
(Urothelial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5563/15822_A_6_5.jpg
(Urothelial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5563/15822_A_6_5.jpg
Figure 8 RNA cell line category: Group enriched (SuSa, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000124731-TREM1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000124731-TREM1
Specifications
- Immunogen
- Mouse myeloma cell line NS0-derived recombinant mouse TREM-1 Ala21-Ser202
- Host Species
- Rat
- Type
- Rat IgG2a
- Specificity
- Trem1
- Species Reactivity
- Mouse
- Applications
- WB, FC, Agonistic assays, CyTOF®, ICC
Product Property
- Purification
- Protein A or G purified
- Purity
- >95%, determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1 µg/mL
FC: 0.25 µg/106 cells
ICC: 8-25 µg/mL
Target
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2466-sdAb | Recombinant Anti-Human TREM1 VHH Single Domain Antibody | ICC, FC, WB, FUNC | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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