Mouse Anti-TSHR Recombinant Antibody (clone 4G11) (CAT#: FAMAB-0323CQ)

Recombinant Mouse Antibody clone 4G11, which is specific to TSHR. The antibody 4G11, selected by the autoantiidiotypic approach following injection of thyrotropin (TSH) into mice, mimic TSH in binding to receptors on thyroid membranes. 4G11 show unequivocal specificity for the TSH receptor.


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ELISA

Figure 1 D2 and  4G11 monoclonal antibodies specifically interact with the TSH receptor on FRTL-6 and TSH receptor-transfected FRT cells.

Figure 1 D2 and 4G11 monoclonal antibodies specifically interact with the TSH receptor on FRTL-6 and TSH receptor-transfected FRT cells.

Panel A, ability of D2 and 4G11 antibody preparations to bind to membrane preparations from FRTL-5 rat thyroid cells as a function of concentration. Binding was measured by an enzyme-linked immunosorbent assay assay. Binding is expressed by the increase in optical density effected by the detection antibody reactivity with substrate resulting in increased absorbance at 415 nm. Data represent the mean of triplicate assays from a single experiment and S.E. is indicated; a duplicate experiment yielded identical data using a different batch of cells. The control IgM in this experiment was the affinity purified mouse IgM from Zymed. The same results were obtained with two monoclonal IgM preparations: IgM, K (Chemicon), and the control IgM used previously. Statistically significant (p < 0.01) binding by comparison to FRT cells with or without normal mouse IgM. Panel B, effect of increasing concentrations of TSH on the binding of D2 and 4G11 to FRTL-5 thyroid membrane preparations. Statistically significant inhibition of binding (p < 0.01). Panel C, binding of D2 and 4G11 antibodies to membranes from FRT cells (FRT cells) which have no functional TSH receptor and no TSH receptor mRNA to membranes from T33 FRT cells (TSH receptor-transfected FRT cells) which contain transfected TSH receptor cDNA and regain TSH binding and TSH-stimulated adenylate cyclase activity, and to membranes from T33 cells treated with 10⁻⁷ M TSH, (TSH-receptor-truncted FRT cells + 10⁻⁷ M TSH). The data represent the mean value of quadruplicate results from a single experiment ± S.E. Two separate experiments using different batches of cells yielded similar results. Where relevant, statistically significant binding (p < 0.01) and inhibition of binding (p < 0.01) by TSH were observed.

Taub, R., Hsu, J. C., Garsky, V. M., Hill, B. L., Erlanger, B. F., & Kohn, L. D. (1992). Peptide sequences from the hypervariable regions of two monoclonal anti-idiotypic antibodies against the thyrotropin (TSH) receptor are similar to TSH and inhibit TSH-increased cAMP production in FRTL-5 thyroid cells. Journal of Biological Chemistry, 267(9), 5977-5984.

ELISA

Figure 2 Ability of peptides to inhibit the binding of the D2 (A) or  4G11 (B) antibodies to membrane preparations from FRTL-5 rat thyroid cells as a function of peptide concentration.

Figure 2 Ability of peptides to inhibit the binding of the D2 (A) or 4G11 (B) antibodies to membrane preparations from FRTL-5 rat thyroid cells as a function of peptide concentration.

Binding was measured by an enzyme-linked immunosorbent assay. Binding is expressed by the increase in optical density effected by the detection antibody reactivity with substrate resulting in increased absorbance at 415 nm. The mean of three separate experiments and the S.E. is indicated. In all experiments, all of the unrelated peptides (in A: P3, P5, P7; in B: PI, P4, P6) were used as controls, although the data for only one unrelated peptide is shown. The x-axis represents a logarithmic scale of peptide concentration.

Taub, R., Hsu, J. C., Garsky, V. M., Hill, B. L., Erlanger, B. F., & Kohn, L. D. (1992). Peptide sequences from the hypervariable regions of two monoclonal anti-idiotypic antibodies against the thyrotropin (TSH) receptor are similar to TSH and inhibit TSH-increased cAMP production in FRTL-5 thyroid cells. Journal of Biological Chemistry, 267(9), 5977-5984.

Inhib

Figure 3 Ability of peptides related to D2 (A) or 4G11 (B) to inhibit the ability of 5 X 10⁻¹⁰ M TSH to increase cAMP levels in rat FRTL-5 thyroid cells.

Figure 3 Ability of peptides related to D2 (A) or 4G11 (B) to inhibit the ability of 5 X 10⁻¹⁰ M TSH to increase cAMP levels in rat FRTL-5 thyroid cells.

Taub, R., Hsu, J. C., Garsky, V. M., Hill, B. L., Erlanger, B. F., & Kohn, L. D. (1992). Peptide sequences from the hypervariable regions of two monoclonal anti-idiotypic antibodies against the thyrotropin (TSH) receptor are similar to TSH and inhibit TSH-increased cAMP production in FRTL-5 thyroid cells. Journal of Biological Chemistry, 267(9), 5977-5984.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgM
  • Specificity
  • Human TSHR
  • Species Reactivity
  • Human
  • Clone
  • 4G11
  • Applications
  • ELISA, Inhib
  • Related Disease
  • Graves' disease

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay and Inhibition.

Target

  • Alternative Names
  • TSHR; thyroid stimulating hormone receptor; LGR3; CHNG1; hTSHR-I; thyrotropin receptor; thyrotropin receptor-I; hTSHR-I; seven transmembrane helix receptor; thyroid stimulating hormone receptor; isoform 2

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Blocking Antibody

CAT Product Name Application Type
NEUT-2234CQ Mouse Anti-TSHR Recombinant Antibody (clone 49) Block, ELISA, ICC, IF, IP, WB Mouse IgG1

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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