Mouse Anti-TSPAN33 Recombinant Antibody (clone 2F8A12) (CAT#: VS7-0425-WR1060)

• Immunogen
• Recombinant human TSPAN33 (AA:Extra(46-64)+Extra(118-235) fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG2b

• Specificity
• Human TSPAN33

• Species Reactivity
• Human

• Clone
• 2F8A12

• Molecule Class
• Tetraspanin

• Applications
• FC

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• 0.05% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• PEN; PEN.; TSPAN-33

• Gene ID
• 340348

• UniProt ID
• Q86UF1

• Long Name
• Tetraspanin 33

• Cellular Localization
• Cell junction; Cell membrane; Cytoplasm; Membrane

• Post Translation Modifications
• Disulfide bond; Glycoprotein

• Protein Refseq
• NP_848657.1

• Function
• Part of TspanC8 subgroup, composed of 6 members that interact with the transmembrane metalloprotease ADAM10. This interaction is required for ADAM10 exit from the endoplasmic reticulum and for enzymatic maturation and trafficking to the cell surface as well as substrate specificity. Different TspanC8/ADAM10 complexes have distinct substrates (PubMed:26686862, PubMed:30463011, PubMed:37516108).; Plays an important role in normal erythropoiesis (By similarity).; It has a role in the differentiation of erythroid progenitors (By similarity).; Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity (PubMed:26686862).; Mediates docking of ADAM10 to zonula adherens by interacting with ADAM10 and, in a PDZD11-dependent manner, with the zonula adherens protein PLEKHA7 (PubMed:30463011).

Isotype Control

• MOB-203CQ
• Mouse IgG2b, kappa Isotype Control Antibody

Secondary Antibody

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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