Mouse Anti-TSPAN33 Recombinant Antibody (clone 8A2A9)
CAT#: VS7-0425-WR1059
This product is a mouse IgG2b antibody that binds to human TSPAN33. The antibody 8A2A9 recognizes specific amino acid domain of human TSPAN33 (AA:Extra(46-64)+Extra(118-235). It can be used for IHC and FC assays.
Specifications
- Immunogen
- Recombinant human TSPAN33 (AA:Extra(46-64)+Extra(118-235) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human TSPAN33
- Species Reactivity
- Human
- Clone
- 8A2A9
- Molecule Class
- Tetraspanin
- Applications
- IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PEN; PEN.; TSPAN-33
- Gene ID
- 340348
- UniProt ID
- Q86UF1
- Long Name
- Tetraspanin 16
- Cellular Localization
- Cell junction; Cell membrane; Cytoplasm; Membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein
- Protein Refseq
- NP_848657.1
- Function
- Part of TspanC8 subgroup, composed of 6 members that interact with the transmembrane metalloprotease ADAM10. This interaction is required for ADAM10 exit from the endoplasmic reticulum and for enzymatic maturation and trafficking to the cell surface as well as substrate specificity. Different TspanC8/ADAM10 complexes have distinct substrates (PubMed:26686862, PubMed:30463011, PubMed:37516108).; Plays an important role in normal erythropoiesis (By similarity).; It has a role in the differentiation of erythroid progenitors (By similarity).; Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity (PubMed:26686862).; Mediates docking of ADAM10 to zonula adherens by interacting with ADAM10 and, in a PDZD11-dependent manner, with the zonula adherens protein PLEKHA7 (PubMed:30463011).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "TSPAN33"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS7-0425-WR1060 | Mouse Anti-TSPAN33 Recombinant Antibody (clone 2F8A12) | FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7577 | Anti-TSPAN33 Immunohistochemistry Kit | IHC |
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