Mouse Anti-TUBA8 Monoclonal Antibody (clone 2D6) (CAT#: VS7-0425-WR1067)

• Immunogen
• Recombinant human TUBA8 fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG2b

• Specificity
• Human TUBA8

• Species Reactivity
• Human, Rat

• Clone
• 2D6

• Molecule Class
• Alpha-Tubulin Protein

• Applications
• WB, IHC, ICC, FC

• Conjugate
• Unconjugated

• Related Disease
• Macrothrombocytopenia, isolated, 2, autosomal dominant (MACTHC2)

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Preservative
• 0.03% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Immunohistochemistry-Paraffin: 1:200 - 1:1000
  Immunocytochemistry: 1:200 - 1:1000
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• TUBA8; Tubulin Alpha-8 Chain; Alpha-Tubulin 8; Tubulin, Alpha 8; TUBAL2

• Gene ID
• 51807

• UniProt ID
• Q9NY65

• Long Name
• Tubulin Alpha 8

• Cellular Localization
• Cytoplasm; Cytoskeleton; Microtubule

• Post Translation Modifications
• Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866).; Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).; Glutamylation is also involved in cilia motility (By similarity).; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally.; The C-terminal phenylalanine residue is cleaved by MATCAP1/KIAA0895L.

• Protein Refseq
• NP_001180343.1

• Function
• Hydrolase

Isotype Control

• MOB-203CQ
• Mouse IgG2b, kappa Isotype Control Antibody

Secondary Antibody

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

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