Mouse Anti-TUBA8 Monoclonal Antibody (clone 2D6)
CAT#: VS7-0425-WR1067
This product is a mouse IgG2b antibody that binds to human TUBA8. The antibody 2D6 can be used for WB, IHC, ICC, and FC assays. It cross reacts with rat.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46031/103368_B_9_5.jpg
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Specifications
- Immunogen
- Recombinant human TUBA8 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human TUBA8
- Species Reactivity
- Human, Rat
- Clone
- 2D6
- Molecule Class
- Alpha-Tubulin Protein
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Macrothrombocytopenia, isolated, 2, autosomal dominant (MACTHC2)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- TUBA8; Tubulin Alpha-8 Chain; Alpha-Tubulin 8; Tubulin, Alpha 8; TUBAL2
- Gene ID
- 51807
- UniProt ID
- Q9NY65
- Long Name
- Tubulin Alpha 8
- Cellular Localization
- Cytoplasm; Cytoskeleton; Microtubule
- Post Translation Modifications
- Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866).; Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).; Glutamylation is also involved in cilia motility (By similarity).; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally.; The C-terminal phenylalanine residue is cleaved by MATCAP1/KIAA0895L.
- Protein Refseq
- NP_001180343.1
- Function
- Hydrolase
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TUBA8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3974z | Mouse Anti-TUBA8 Recombinant Antibody (clone 20B3) | WB, FC, ELISA, IF, IP, IHC | Mouse IgG2a, κ |
| VS3-QX1192 | Mouse Anti-TUBA8 Recombinant Antibody (clone 3B12-F1-C2) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY519 | Mouse Anti-NHP TUBA8 Recombinant Antibody (clone OTI2C2) | WB, ICC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7610 | Anti-TUBA8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7611 | Anti-Mouse TUBA8 Immunohistochemistry Kit | IHC |
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