CytoStream™ Rabbit Anti-TUBB Recombinant Antibody (VS13-YC1191)
CAT#: VS13-YC1191
The CytoStream™ Rabbit Anti-TUBB Recombinant Antibody is specific for human, mouse and rat TUBB. The TUBB gene encodes a beta-tubulin protein that forms a dimer with alpha-tubulin, acting as a structural component of microtubules. Mutations in this gene can lead to cortical dysplasia and complex brain malformations. The anti-TUBB antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5417/968_C12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules & cytokinetic bridge.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43640/1773_E7_6_selected.jpg
Normal Tissue
Figure 3 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5417/ihc_selected.jpg
Normal Tissue
Figure 4 Ovary.
High expression in ovary
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12406/31977_A_5_7_val_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5417/19754_A_8_8.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12406/31977_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12406/31977_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12406/31977_A_5_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196230-TUBB
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Specifications
- Immunogen
- Recombinant human beta Tubulin, aa 308-357.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat TUBB
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/5000
Immunocytochemistry (ICC): 1/50
Immunohistochemistry-paraffin (IHC-P): 1/100-1/400
Flow cytometry (FC): 1/50
Target
- Alternative Names
- Tubulin Beta Class I; Tubulin, Beta Polypeptide; Tubulin Beta-5 Chain; TUBB5; Tubulin, Beta Class I; Class I Beta-Tubulin; Tubulin Beta-1 Chain; Tubulin Beta Chain; Beta Ib Tubulin;
- Gene ID
- 203068
- UniProt ID
- P07437
- Long Name
- Tubulin Beta Class I
- Sequence Similarities
- Belongs to the tubulin family.
- Cellular Localization
- Cytoplasm
- Protein Refseq
- NP_001280141.1; NP_001280142.1; NP_001280143.1
- Function
- Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "TUBB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3721 | Rabbit Anti-TUBB Recombinant Antibody (clone DS3721AB) | WB, IHC, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0816LC | Mouse Anti-TUBB Recombinant Antibody (clone TU-06) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0204YJ | Rabbit Anti-TUBB Polyclonal Antibody | WB, IHC, IF, ICC, ELISA | Rabbit IgG |
| MRO-0206YJ | Mouse Anti-TUBB Recombinant Antibody (clone CBS162) | WB, IHC, IF, ICC, ELISA | Mouse IgG |
| VS3-CJ469 | Mouse Anti-TUBB Recombinant Antibody (VS3-CJ469), HRP | WB, IHC | Mouse IgG |
| VS3-CJ470 | Mouse Anti-TUBB Recombinant Antibody (VS3-CJ470) | WB, IF, IHC-P | Mouse IgG2b, κ |
| VS3-CJ471 | Rabbit Anti-TUBB Recombinant Antibody (VS3-CJ471) | WB, ICC, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7612 | Anti-TUBB Immunohistochemistry Kit | IHC | |
| VS-0525-XY7613 | Anti-Mouse TUBB Immunohistochemistry Kit | IHC | |
| VS-0525-XY7614 | Anti-Rat TUBB Immunohistochemistry Kit | IHC | |
| VS-0525-XY7615 | Anti-Chicken TUBB Immunohistochemistry Kit | IHC |
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