CytoStream™ Rabbit Anti-UBB Recombinant Antibody (VS13-YC1200) (CAT#: VS13-YC1200)

• Immunogen
• Recombinant human Ubiquitin, aa 90-120.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat UBB

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ICC, IF, IHC-P, FC

• Conjugate
• NONE

• Related Disease
• Alzheimer's disease and Down syndrome

• Clonality
• Monoclonal

• Purification
• Protein A purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.

• Preservative
• 0.05% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/500
  Immunocytochemistry (ICC): 1/50-1/200
  Immunofluorescence (IF): 1/50-1/200
  Immunohistochemistry-paraffin (IHC-P): 1/200-1/500
  Flow cytometry (FC): 1/50-1/100

• Alternative Names
• Ubiquitin B; Polyubiquitin B; Epididymis Secretory Protein Li 50; Polyubiquitin-B; HEL-S-50;

• Gene ID
• 7314

• UniProt ID
• P0CG47

• Long Name
• Ubiquitin B

• Sequence Similarities
• Belongs to the ubiquitin family.

• Cellular Localization
• Nucleus; Cytoplasm; Cell membrane; Mitochondrion

• Protein Refseq
• NP_001268645.1; NP_001268646.1; NP_001268647.1N

• Function
• Ubiquitin:
  Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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