Rabbit Anti-UBE2A Recombinant Antibody (clone R87-4N-5)
CAT#: VS3-FY2137
This is a ubiquitin conjugating enzyme E2 A/B rabbit recombinant monoclonal antibody. It demonstrates high specificity in target human UBE2A. The R87-4N-5 is recommended for dilution with western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunofluorescence (dilution 1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51765/769_F12_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/65898/1247_B7_3_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000077721-UBE2A
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human UBE2A
- Species Reactivity
- Human
- Clone
- R87-4N-5
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 17 kDa, while the observed molecular weight stands at 17kDa.
- Related Disease
- Intellectual Developmental Disorder, X-Linked, Syndromic, Nascimento Type and Syndromic X-Linked Intellectual Disability Nascimento Type
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Ubiquitin Conjugating Enzyme E2 A; E2 Ubiquitin-Conjugating Enzyme A; Ubiquitin-Conjugating Enzyme E2A; Ubiquitin Carrier Protein A; Ubiquitin-Protein Ligase A; RAD6 Homolog A; HHR6A; RAD6A; Ubiquitin-Conjugating Enzyme E2A (RAD6 Homolog)
- Gene ID
- 7319
- UniProt ID
- P49459
- Sequence Similarities
- Belongs to the ubiquitin-conjugating enzyme family.
- Cellular Localization
- cytosol, nucleoplasm
- Post Translation Modifications
- Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B. (P49459-UBE2A_HUMAN)
Ubiquitination at Lys75 (NX_P49459 [NX_P49459-1])
Modification sites at PhosphoSitePlus (P49459)
- Protein Refseq
- NP_001269090.1; NP_003327.2; NP_861427.1
- Function
- Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "UBE2A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0270YC | Human Anti-UBE2A Recombinant Antibody (clone AA2) | IF, IP, WB | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0270-YC-S(P) | Human Anti-UBE2A Recombinant Antibody (clone AA2); scFv Fragment | IF, IP, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0270-YC-F(E) | Human Anti-UBE2A Recombinant Antibody (clone AA2); Fab Fragment | IF, IP, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY269 | AbPlus™ Anti-UBE2A Magnetic Beads (AA2) | IP, Protein Purification |
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