Rabbit Anti-UBE2A Recombinant Antibody (clone R87-4N-5) (CAT#: VS3-FY2137)
This is a ubiquitin conjugating enzyme E2 A/B rabbit recombinant monoclonal antibody. It demonstrates high specificity in target human UBE2A. The R87-4N-5 is recommended for dilution with western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunofluorescence (dilution 1/50-1/200).
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Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51765/769_F12_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51765/769_F12_2_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/65898/1247_B7_3_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/65898/1247_B7_3_selected.jpg
Figure 3 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000077721-UBE2A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000077721-UBE2A
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human UBE2A
- Species Reactivity
- Human
- Clone
- R87-4N-5
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 17 kDa, while the observed molecular weight stands at 17kDa.
- Related Disease
- Intellectual Developmental Disorder, X-Linked, Syndromic, Nascimento Type and Syndromic X-Linked Intellectual Disability Nascimento Type
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Ubiquitin Conjugating Enzyme E2 A; E2 Ubiquitin-Conjugating Enzyme A; Ubiquitin-Conjugating Enzyme E2A; Ubiquitin Carrier Protein A; Ubiquitin-Protein Ligase A; RAD6 Homolog A; HHR6A; RAD6A; Ubiquitin-Conjugating Enzyme E2A (RAD6 Homolog)
- Gene ID
- 7319
- UniProt ID
- P49459
- Sequence Similarities
- Belongs to the ubiquitin-conjugating enzyme family.
- Cellular Localization
- cytosol, nucleoplasm
- Post Translation Modifications
- Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B. (P49459-UBE2A_HUMAN)
Ubiquitination at Lys75 (NX_P49459 [NX_P49459-1])
Modification sites at PhosphoSitePlus (P49459)
- Protein Refseq
- NP_001269090.1; NP_003327.2; NP_861427.1
- Function
- Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0270YC | Human Anti-UBE2A Recombinant Antibody (clone AA2) | IF, IP, WB | Human IgG |
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