Mouse Anti-UBE2C Recombinant Antibody (VS3-CJ117) (CAT#: VS3-CJ117)
This product is a mouse antibody that recognizes UBE2C (C-term).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34569/1216_F6_1_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34569/1216_F6_1_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/265_D2_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/265_D2_2_selected.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54975/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54975/ihc_selected.jpg
Figure 4 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11464/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11464/ihc_selected.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_8.jpg
Figure 7 Tonsil
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_8.jpg
Figure 8 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_3.jpg
Figure 9 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_4.jpg
Figure 10 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_5.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175063-UBE2C
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175063-UBE2C
Specifications
- Immunogen
- KLH-conjugated synthetic peptide between 137~172 amino acids from the C-terminal region of human UBE2C
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human UBE2C
- Species Reactivity
- Human
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A/G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:2000).
Target
- Alternative Names
- UBCH10; dJ447F3.2
- Gene ID
- 11065
- UniProt ID
- O00762
- Sequence Similarities
- Belongs to the ubiquitin-conjugating enzyme family.
- Cellular Localization
- Cytosol, Nucleoplasm, Nucleus, Plasma membrane
- Post Translation Modifications
- Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.
- Protein Refseq
- NP_001268670.1; NP_001268671.1; NP_008950.1
- Function
- Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
Recommended Products
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3665z | Mouse Anti-UBE2C Recombinant Antibody (clone 16G12) | WB, IHC, IF, ELISA | Mouse IgG1 |
| MOB-1197MZ | Recombinant Mouse Anti-Human UBE2C Antibody | IHC-P, WB | Mouse antibody |
| FAMAB-1285CQ | Mouse Anti-UBE2C Recombinant Antibody (clone SAIC-41C-6) | Immuno-MRM, WB | Mouse IgG1 |
| FAMAB-1286CQ | Mouse Anti-UBE2C Recombinant Antibody (clone SAIC-41A-1) | Immuno-MRM, WB, ELISA | Mouse IgG1 |
| VS3-FY1511 | Recombinant Rabbit Anti-UBE2C Antibody (clone R08-4F5) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
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