Mouse Anti-Ubiquitin Recombinant Antibody (clone 5F1) (CAT#: ZG-0165C)
This product is a mouse antibody that recognizes Ubiquitin. It can be used in a wide variety of applications, such as: WB, IF/ICC, IHC-P.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Mouse Anti-Ubiquitin Antibody (clone 5F1) in IHC
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, Ubiquitin Mouse Monoclonal Antibody (5F1) was diluted at 1:200 (4° C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98° C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
Figure 2 Mouse Anti-Ubiquitin Antibody (clone 5F1) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Ubiquitin Mouse Monoclonal Antibody (5F1) was diluted at 1:200 (4° C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98° C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
Figure 3 Mouse Anti-Ubiquitin Antibody (clone 5F1) in IF
Immunofluorescence analysis of Human-stomach-cancer tissue. 1, Ubiquitin Mouse Monoclonal Antibody (5F1) (red) was diluted at 1:200 (4° C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Figure 4 Mouse Anti-Ubiquitin Antibody (clone 5F1) in IF
Immunofluorescence analysis of Mouse-brain tissue. 1, Ubiquitin Mouse Monoclonal Antibody (5F1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Figure 5 Mouse Anti-Ubiquitin Antibody (clone 5F1) in IF
Immunofluorescence analysis of Rat-brain tissue. 1, Ubiquitin Mouse Monoclonal Antibody (5F1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Figure 6 Mouse Anti-Ubiquitin Antibody (clone 5F1) in IHC
Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200.
Figure 7 Mouse Anti-Ubiquitin Antibody (clone 5F1) in IHC
Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200.
Figure 8 Mouse Anti-Ubiquitin Antibody (clone 5F1) in WB
WB analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using Ubiquitin Mouse mAb diluted at 1:1000.
Specifications
- Immunogen
- Synthetic Peptide of Ubiquitin
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Ubiquitin
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 5F1
- Applications
- WB, IF/ICC, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunofluorescence, Immunohistochemistry-Paraffin. For details, refer to Tested Data.
Target
- Alternative Names
- Ubiquitin
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Ubiquitin"
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1022 | Recombinant Anti-Ubiquitin Antibody | ELISA, WB, Neut, FuncS | IgG |
MHH-1022 | Recombinant Human Anti-Ubiquitin Antibody | RIA, IP, FuncS | IgG |
ZG-0166C | Mouse Anti-Ubiquitin Recombinant Antibody (clone 6G6) | WB, IHC-P, IF | Mouse IgG |
ZG-460C | Mouse Anti-Ubiquitin Recombinant Antibody (clone C1) | ELISA, WB | Mouse IgG2a |
VS-0224-XY137 | Mouse Anti-Ubiquitin Recombinant Antibody (VS-0224-XY137) | ELISA, WB | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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