AbPlus™ Anti-UCHL1 Magnetic Beads (JF93-08) (CAT#: VS-0424-XY270)
The AbPlus Anti-UCHL1 Magnetic Beads (JF93-08) is an innovative affinity resin which is bound with anti-UCHL1 specific antibody (JF93-08). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of UCHL1 protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5993/1902_A11_2_selected.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5993/1902_A11_2_selected.jpg

Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/665_E11_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/665_E11_1_selected.jpg

Figure 3 Kidney
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_8_5.jpg
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_8_5.jpg

Figure 4 Testis
(Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_6_6.jpg
(Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_6_6.jpg

Figure 5 Cerebral cortex
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_B_8_5.jpg
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_B_8_5.jpg

Figure 6 Colon
(Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25% Peripheral nerve/ganglion Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_9_3.jpg
(Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25% Peripheral nerve/ganglion Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_9_3.jpg

Figure 7 Pancreas
(Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_1_3.jpg
(Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_1_3.jpg

Figure 8 RNA cell line category: Cell line enhanced (EFO-21, SCLC-21H, U-251 MG, U-266/84, U-87 MG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000154277-UCHL1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000154277-UCHL1
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-UCHL1 specific antibody (JF93-08)
- Target
- UCHL1
- Immunogen
- N terminus of human UCHL1
- Target Species
- Human, Mouse, Rat
- Antibody Clone
- JF93-08
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.
- Alternative Names
- Ubiquitin C-Terminal Hydrolase L1; Ubiquitin Carboxyl-Terminal Esterase L1 (Ubiquitin Thiolesterase); Neuron Cytoplasmic Protein 9.5; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase; PGP 9.5; Uch-L1; PGP9.5; Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1; Epididymis Secretory Protein Li 53
- Gene ID
- 7345
- UniProt ID
- P09936
Downloads
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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