Mouse Anti-UCHL1 Recombinant Antibody (MOB-0186F)
CAT#: MOB-0186F
This product is a mouse antibody that recognizes UCHL1.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-UCHL1 Recombinant Antibody (MOB-0186F) in IHC
Immunohistochemical analysis of paraffin-embedded Kindey. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Use pH8.0 TRIS-EDTA for antigen retrieval. 3. Dilute the secondary antibody at 1:200 (room temperature, 30min).
IHC
Figure 2 Mouse Anti-UCHL1 Recombinant Antibody (MOB-0186F) in IHC
Immunohistochemical analysis of paraffin-embedded Kindey. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Use pH8.0 TRIS-EDTA for antigen retrieval. 3. Dilute the secondary antibody at 1:200 (room temperature, 30min).
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Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5993/1902_A11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/665_E11_1_selected.jpg
Normal Tissue
Figure 3 Kidney
Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_6_6.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25% Peripheral nerve/ganglion Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_9_3.jpg
Normal Tissue
Figure 7 Pancreas
Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_1_3.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (EFO-21, SCLC-21H, U-251 MG, U-266/84, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000154277-UCHL1
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Specifications
- Immunogen
- Synthesized peptide derived from human PGP 9.5
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human UCHL1
- Species Reactivity
- Human, Mouse
- Applications
- IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistologyp-Paraffin Sections, Immunofluorescence.
Target
- Alternative Names
- Ubiquitin C-Terminal Hydrolase L1; Ubiquitin Carboxyl-Terminal Esterase L1 (Ubiquitin Thiolesterase); Neuron Cytoplasmic Protein 9.5; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase; PGP 9.5; Uch-L1; PGP9.5; Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1; Epididymis Secretory Protein Li 53
- Gene ID
- 7345
- UniProt ID
- P09936
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "UCHL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1897MZ | Recombinant Mouse Anti-Human UCHL1 Antibody (clone 20B2) | IHC-Fr, IHC-P, WB | Mouse antibody |
| MRO-1216-CN | Mouse Anti-UCHL1 Recombinant Antibody (clone B1-5-6) | WB, IHC, IF, FC | Mouse IgG2a |
| MRO-1217-CN | Rabbit Anti-UCHL1 Recombinant Antibody (clone JF93-08) | WB, IF, IHC, IP | Rabbit IgG |
| MRO-1218-CN | Rabbit Anti-UCHL1 Recombinant Antibody (clone JM10-59) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-QX1215 | Mouse Anti-UCHL1 Recombinant Antibody (clone 7B8-G5-E7) | WB, ICC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0615MZ | Chicken Anti-UCHL1 Polyclonal IgY | WB | Chicken antibody |
| BRD-0800MZ | Chicken Anti-PGP9.5 Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3771 | Rabbit Anti-UCHL1 Recombinant Antibody (clone DS3771AB) | WB, IP, IHC-P, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY270 | AbPlus™ Anti-UCHL1 Magnetic Beads (JF93-08) | IP, Protein Purification | |
| VS-0724-YC1162 | AbPlus™ Anti-Uchl1 Magnetic Beads (VS-0724-YC1162) | IP, Protein Purification | |
| VS-0724-YC1164 | AbPlus™ Anti-UCHL1 Magnetic Beads (VS-0724-YC1164) | IP, Protein Purification | |
| VS-0724-YC1237 | AbPlus™ Anti-Uchl1 Magnetic Beads (VS-0724-YC1237) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2427 | Anti-UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7722 | Anti-Mouse UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7723 | Anti-Rat UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7721 | Anti-Human UCHL1 Immunohistochemistry Kit | IHC |
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