Mouse Anti-UCHL1 Recombinant Antibody (VS3-FY2912)
CAT#: VS3-FY2912
The anti-UCHL1 antibody is a mouse recombinant monoclonal antibody. It has been specifically designed to target the human UCHL1 protein. It cross-reacts with monkey species. The monoclonal antibody can be used in the area of Spastic Paraplegia 79B, Autosomal Recessive and Parkinson Disease 5, Autosomal Dominant.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5993/1902_A11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/665_E11_1_selected.jpg
Normal Tissue
Figure 3 Kidney
Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_6_6.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25% Peripheral nerve/ganglion Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_9_3.jpg
Normal Tissue
Figure 7 Pancreas
Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_1_3.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (EFO-21, SCLC-21H, U-251 MG, U-266/84, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000154277-UCHL1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human and Monkey UCHL1
- Species Reactivity
- Human, Monkey
- Applications
- Western Blot, Immunocytochemistry
- Conjugate
- Unconjugated, FITC
- Related Disease
- Spastic Paraplegia 79B, Autosomal Recessive and Parkinson Disease 5, Autosomal Dominant
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunocytochemistry: 1/50-1/200
Target
- Alternative Names
- Ubiquitin C-Terminal Hydrolase L1; Ubiquitin Carboxyl-Terminal Esterase L1 (Ubiquitin Thiolesterase); Neuron Cytoplasmic Protein 9.5; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase; PGP 9.5; Uch-L1; PGP9.5; Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1; Epididymis Secretory Protein Li 53
- Gene ID
- 7345
- UniProt ID
- P09936
- Sequence Similarities
- Belongs to the peptidase C12 family.
- Cellular Localization
- Cytoplasm, Endoplasmic reticulum, Membrane
- Post Translation Modifications
- O-glycosylated
- Protein Refseq
- NP_004172.2
- Function
- Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "UCHL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0615MZ | Chicken Anti-UCHL1 Polyclonal IgY | WB | Chicken antibody |
| BRD-0800MZ | Chicken Anti-PGP9.5 Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3771 | Rabbit Anti-UCHL1 Recombinant Antibody (clone DS3771AB) | WB, IP, IHC-P, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0493F | Mouse Anti-UCHL1 Recombinant Antibody (ZG-0493F) | WB, ICC | Mouse IgG |
| ZG-0494F | Mouse Anti-UCHL1 Recombinant Antibody (ZG-0494F) | WB, ICC | Mouse IgG |
| VS3-XY3533 | Rabbit Anti-UCHL1 Recombinant Antibody (clone R07-2E5) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| VS3-WK1908 | Recombinant Rabbit Anti-UCHL1 Capture Antibody (clone R18-1C-8) | ELISA | Rabbit IgG |
| VS3-WK1909 | Recombinant Rabbit Anti-UCHL1 Detection Antibody (clone R37-3A-8) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY270 | AbPlus™ Anti-UCHL1 Magnetic Beads (JF93-08) | IP, Protein Purification | |
| VS-0724-YC1162 | AbPlus™ Anti-Uchl1 Magnetic Beads (VS-0724-YC1162) | IP, Protein Purification | |
| VS-0724-YC1164 | AbPlus™ Anti-UCHL1 Magnetic Beads (VS-0724-YC1164) | IP, Protein Purification | |
| VS-0724-YC1237 | AbPlus™ Anti-Uchl1 Magnetic Beads (VS-0724-YC1237) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2427 | Anti-UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7722 | Anti-Mouse UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7723 | Anti-Rat UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7721 | Anti-Human UCHL1 Immunohistochemistry Kit | IHC |
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