Rabbit Anti-ULK3 Recombinant Antibody (clone R08-7J3)
CAT#: VS3-FY1930
This anti-ULK3 antibody detected positive in western blot analysis of ULK3 in K562, Hela lysates. The recombinant monoclonal antibody demonstrates high specificity in target human ULK3. This R08-7J3 antibody is validated for western blot analysis of ULK3 in K562, Hela lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-ULK3 Antibody (VS3-FY1930) in WB.
The ULK3 expression in K562, Hela lysates were detected using the anti-ULK3 by western blot assay.
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Normal Tissue
Figure 1 IHC staining of human colon
Immunohistochemical staining of human colon shows distinct cytoplasmic positivity with a granular pattern in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40474/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40474/108923_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40474/108923_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40474/108923_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40474/108923_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40474/108923_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40474/108923_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140474-ULK3
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Specifications
- Immunogen
- Synthetic peptide of human ULK3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ULK3
- Species Reactivity
- Human
- Clone
- R08-7J3
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 53 kDa, while the observed molecular weight stands at 53 kDa.
- Related Disease
- Non-Syndromic X-Linked Intellectual Disability 97
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Unc-51 Like Kinase 3; Unc-51-Like Kinase 3 (C. Elegans); Unc-51-Like Kinase 3; EC 2.7.11.1
- Gene ID
- 25989
- UniProt ID
- Q6PHR2
- Sequence Similarities
- Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Autophosphorylated.
Autophosphorylation is blocked by interaction with SUFU.
(Q6PHR2-ULK3_HUMAN)
Ubiquitination at Lys345 (NX_Q6PHR2 [NX_Q6PHR2-1])
Modification sites at PhosphoSitePlus (Q6PHR2)
- Protein Refseq
- XP_005254346.1; XP_016877557.1; XP_047288324.1; XP_047288325.1; XP_047288326.1
- Function
- Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ULK3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3789 | Rabbit Anti-ULK3 Recombinant Antibody (clone DS3789AB) | WB, IHC, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1220 | Mouse Anti-ULK3 Recombinant Antibody (clone 1404CT148.89.25) | WB | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
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| VS3-WK806 | Rabbit Anti-ULK3 Recombinant Antibody (clone VS3-WK806) | WB, IHC, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1035 | Mouse Anti-ULK3 Recombinant Antibody (VS3-WK1035) | WB | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1522 | Recombinant Mouse Anti-ULK3 Antibody (clone 5A5G2) | ELISA, WB, IHC, ICC, FC | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7744 | Anti-ULK3 Immunohistochemistry Kit | IHC |
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