Rabbit Anti-ULK3 Recombinant Antibody (clone R08-7J3) (CAT#: VS3-FY1930)

• Immunogen
• Synthetic peptide of human ULK3

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human ULK3

• Species Reactivity
• Human

• Clone
• R08-7J3

• Applications
• Western Blot

• MW
• The calculated weight of the target protein is approximately 53 kDa, while the observed molecular weight stands at 53 kDa.

• Related Disease
• Non-Syndromic X-Linked Intellectual Disability 97

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Unc-51 Like Kinase 3; Unc-51-Like Kinase 3 (C. Elegans); Unc-51-Like Kinase 3; EC 2.7.11.1

• Gene ID
• 25989

• UniProt ID
• Q6PHR2

• Sequence Similarities
• Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

• Cellular Localization
• Cytoplasm

• Post Translation Modifications
• Autophosphorylated.
  Autophosphorylation is blocked by interaction with SUFU.
  (Q6PHR2-ULK3_HUMAN)
  Ubiquitination at Lys345 (NX_Q6PHR2 [NX_Q6PHR2-1])
  Modification sites at PhosphoSitePlus (Q6PHR2)

• Protein Refseq
• XP_005254346.1; XP_016877557.1; XP_047288324.1; XP_047288325.1; XP_047288326.1

• Function
• Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody