Rabbit Anti-USP14 Recombinant Antibody (clone R09-8E9) (CAT#: VS3-XY3536)
It is a recombinant rabbit antibody that specifically recognizes human USP14 (Ubiquitin specific peptidase 14), which plays a role in the innate immune defense against viruses. The antibody also has cross-reactivity against mouse or rat USP14. It has been reported for use in Western Blot, Flow cytometry, Immunocytochemistry or Immunofluorescence.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/59_C4_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/59_C4_2_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_B_8_5.jpg
Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_9_3.jpg
Figure 4 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_8_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_8_4.jpg
Figure 5 Pancreas
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_2_3.jpg
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_2_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_9_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_9_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_5_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_5_6.jpg
Figure 8 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_8_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101557-USP14
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101557-USP14
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat USP14
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-8E9
- Applications
- WB, ICC, IF, FC
- Related Disease
- Machado-Joseph Disease; Autosomal Dominant Cerebellar Ataxia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- TGT; Ubp6
- Gene ID
- 9097
- UniProt ID
- P54578
- Long Name
- Ubiquitin specific peptidase 14
- Sequence Similarities
- Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Proteasome
- Post Translation Modifications
- Ubiquitination at Lys49, Lys62, Lys130, Lys214, Lys291, Lys300, Lys348, Lys441, Lys443, and Lys449 (NX_P54578 [NX_P54578-1])
Modification sites at PhosphoSitePlus (P54578)
Glycosylation from GlyGen (P54578) 10 sites, 1 O-linked glycan (10 sites)
- Protein Refseq
- NP_001032411.1; NP_005142.1
- Function
- Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome.
Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell (PubMed:18162577).
Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "USP14"
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2173CT | Recombinant Mouse anti-Human USP14 Monoclonal antibody (EML864) | WB | |
| MRO-1562-CN | Rabbit Anti-USP14 Recombinant Antibody (clone JU30-49) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX1222 | Mouse Anti-USP14 Recombinant Antibody (clone 889CT6.2.1) | WB, FC | Mouse IgG1 |
| VS3-CJ1037 | Mouse Anti-USP14 Recombinant Antibody (VS3-CJ1037) | WB, FC | Mouse IgG1 |
| VS4-CJ150 | Recombinant Rabbit Anti-USP14 Antibody (clone 28C4) | ELISA, WB, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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