Rabbit Anti-USP14 Recombinant Antibody (clone R09-8E9)
CAT#: VS3-XY3536
It is a recombinant rabbit antibody that specifically recognizes human USP14 (Ubiquitin specific peptidase 14), which plays a role in the innate immune defense against viruses. The antibody also has cross-reactivity against mouse or rat USP14. It has been reported for use in Western Blot, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/59_C4_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_8_4.jpg
Normal Tissue
Figure 5 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_2_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1308/3920_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101557-USP14
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat USP14
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-8E9
- Applications
- WB, ICC, IF, FC
- Related Disease
- Machado-Joseph Disease; Autosomal Dominant Cerebellar Ataxia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- TGT; Ubp6
- Gene ID
- 9097
- UniProt ID
- P54578
- Long Name
- Ubiquitin specific peptidase 14
- Sequence Similarities
- Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Proteasome
- Post Translation Modifications
- Ubiquitination at Lys49, Lys62, Lys130, Lys214, Lys291, Lys300, Lys348, Lys441, Lys443, and Lys449 (NX_P54578 [NX_P54578-1])
Modification sites at PhosphoSitePlus (P54578)
Glycosylation from GlyGen (P54578) 10 sites, 1 O-linked glycan (10 sites)
- Protein Refseq
- NP_001032411.1; NP_005142.1
- Function
- Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome.
Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell (PubMed:18162577).
Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-XY3536. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "USP14"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2173CT | Recombinant Mouse anti-Human USP14 Monoclonal antibody (EML864) | WB | |
| MRO-1562-CN | Rabbit Anti-USP14 Recombinant Antibody (clone JU30-49) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX1222 | Mouse Anti-USP14 Recombinant Antibody (clone 889CT6.2.1) | WB, FC | Mouse IgG1 |
| VS3-CJ1037 | Mouse Anti-USP14 Recombinant Antibody (VS3-CJ1037) | WB, FC | Mouse IgG1 |
| VS4-CJ150 | Recombinant Rabbit Anti-USP14 Antibody (clone 28C4) | ELISA, WB, IF | Rabbit IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: WB, ELISA, FC, IHC, IP
Application: ELISA
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FC, IHC, Neut
Application: ELISA, IF, Block, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
