Rabbit Anti-USP24 Recombinant Antibody (clone R43-4X-7) (CAT#: VS3-FY2141)
This R43-4X-7 recombinant monoclonal antibody specifically binds to human USP24 protein, a calculated weight of 294 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R43-4X-7 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26723/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26723/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28428/497_B9_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28428/497_B9_2_selected.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67124/1237_G5_3_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67124/1237_G5_3_selected.jpg
Figure 4 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26723/60247_A_7_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26723/60247_A_7_5.jpg
Figure 5 Stomach
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26723/60247_A_6_1.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26723/60247_A_6_1.jpg
Figure 6 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26723/60247_A_9_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26723/60247_A_9_3.jpg
Figure 7 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26723/60247_A_9_4.jpg
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26723/60247_A_9_4.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162402-USP24
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162402-USP24
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat USP24
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R43-4X-7
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 294 kDa, while the observed molecular weight stands at 294kDa.
- Related Disease
- Non-Syndromic X-Linked Intellectual Disability 99 and Parkinson Disease, Late-Onset
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Ubiquitin Specific Peptidase 24; Ubiquitin-Specific-Processing Protease 24; Ubiquitin Specific Protease 24; Deubiquitinating Enzyme 24; Ubiquitin Thioesterase 24; EC 3.4.19.12; KIAA1057
- Gene ID
- 23358
- UniProt ID
- Q9UPU5
- Sequence Similarities
- Belongs to the peptidase C19 family.
- Cellular Localization
- Cytosol, Nucleoplasm, Nucleus
- Protein Refseq
- NP_056121.2
- Function
- Ubiquitin-specific protease that regulates cell survival in various contexts through modulating the protein stability of some of its substrates including DDB2, MCL1 or TP53. Plays a positive role on ferritinophagy where ferritin is degraded in lysosomes and releases free iron.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3806 | Rabbit Anti-USP24 Recombinant Antibody (clone DS3806AB) | WB | Rabbit IgG |
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