Chicken Anti-USUV E DIII Recombinant Antibody (clone G1) (CAT#: VS-0323-CJ66)
This chicken anti-USUV E DIII antibody (clone G1) is a product that recognizes Usutu virus (USUV) envelope protein domain III.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Binding of scFv to E protein DIII antigens in an ELISA.
The E protein DIII antigens of USUV A, B, I and WNV, were coated on high-binding ELISA plates. BSA in PBS was used as a negative control.
Figure 2 Western blot titrations of VS-0323-CJ66 using E protein DIII of USUV as antigens.
Immunoblots of decreasing amounts of USUV E protein DIII from groups A, B, and I E protein DIII were incubated with the VS-0323-CJ66 at a dilution of 1:2,000, followed by mouse anti-HA antibodies, and HRP-conjugated goat anti-mouse antibodies for visualization.
Figure 3 Western blots with E protein DIII and sE of USUV and WNV as antigens.
Western blots of DIII from the E protein and sE (the soluble ectodomain of protein E) proteins of USUV and WNV were incubated with VS-0323-CJ66 at a dilution of 1:2,000, followed by an incubation with mouse anti-HA antibodies and HRP-conjugated goat anti-mouse antibodies.
Figure 4 Virus ELISA binding assay on USUV Vienna‐2001.
A virus ELISA was performed with all five scFvs (VS-0323-CJ63, VS-0323-CJ64, VS-0323-CJ65, VS-0323-CJ66, A9) in two-fold dilution steps (2.5-20 μg/ml).
Specifications
- Immunogen
- Purified Usutu virus envelope protein domain III
- Host Species
- Chicken
- Type
- Chicken IgY
- Specificity
- Usutu virus (USUV) envelope protein domain III
- Species Reactivity
- USUV
- Clone
- G1
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Surface Plasmon Resonance, Neutralization
- Conjugate
- Unconjugated
- Related Disease
- Encephalitis
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
ELISA: 2.5 μg/mL-20 μg/mL
Target
- Alternative Names
- Usutu virus envelope protein domain III
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products
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CAT | Product Name | Application | Type |
---|---|---|---|
VS-0323-CJ63 | Chicken Anti-USUV E DIII Recombinant Antibody (clone A8) | ELISA, WB, SPR, Neut | Chicken IgY |
VS-0323-CJ64 | Chicken Anti-USUV E DIII Recombinant Antibody (clone A12) | ELISA, WB, SPR, Neut | Chicken IgY |
VS-0323-CJ65 | Chicken Anti-USUV E DIII Recombinant Antibody (clone D2.2) | ELISA, WB, SPR, Neut | Chicken IgY |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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