Human Anti-αVβ6 integrin Recombinant Antibody (clone 264RAD) (CAT#: HPAB-0215LY)
This product is an antibody that recognizes αVβ6 integrin. The antibody 264RAD can be used for immunoassay techniques such as: ELISA, FC.
Specific Inquiry
Inhib
Figure 1 264RAD binds and inhibits αvβ6 integrin.
Binding of K562 cells overexpressing αvβ6 to wells coated with 10 μg/ml human IgG1 control, 264RAD, 4B4 and 10D5 antibodies.
Eberlein, C., Kendrew, J., McDaid, K., Alfred, A., Kang, J. S., Jacobs, V. N., ... & Cao, A. (2013). A human monoclonal antibody 264RAD targeting αvβ6 integrin reduces tumour growth and metastasis, and modulates key biomarkers in vivo. Oncogene, 32(37), 4406-4416.
FuncS
Figure 2 Cross-species binding and functional activity of 264RAD.
Binding to K562 cells overexpressing human, murine and cynomolgus monkey was assessed by FACS. 264RAD and isotype control antibody were incubated with cells at 15 μg/ml, at RT for 30 min. The geomean for each is shown. The data are representative of more than two experiments. To assess functional activity, the ability of 264RAD to inhibit cell binding to GST TGF-b LAP was assessed.
Eberlein, C., Kendrew, J., McDaid, K., Alfred, A., Kang, J. S., Jacobs, V. N., ... & Cao, A. (2013). A human monoclonal antibody 264RAD targeting αvβ6 integrin reduces tumour growth and metastasis, and modulates key biomarkers in vivo. Oncogene, 32(37), 4406-4416.
Inhib
Figure 3 Inhibition of αvβ8 integrin.
Binding of 264RAD to αvβ8, K562 cells overexpressing αvβ8 were incubated with 264RAD at 10 μg/ml and binding detected by FACS.
Eberlein, C., Kendrew, J., McDaid, K., Alfred, A., Kang, J. S., Jacobs, V. N., ... & Cao, A. (2013). A human monoclonal antibody 264RAD targeting αvβ6 integrin reduces tumour growth and metastasis, and modulates key biomarkers in vivo. Oncogene, 32(37), 4406-4416.
FC
Figure 4 Evaluation of a panel of NSCLC cells to activate αSMA expression in co-cultured NHDF.
Expression of αvβ6 and/or αvβ8 by FACS analysis using 264RAD or IgG.
Eberlein, C., Kendrew, J., McDaid, K., Alfred, A., Kang, J. S., Jacobs, V. N., ... & Cao, A. (2013). A human monoclonal antibody 264RAD targeting αvβ6 integrin reduces tumour growth and metastasis, and modulates key biomarkers in vivo. Oncogene, 32(37), 4406-4416.
Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG1
- Specificity
- Human and Monkey αVβ6 integrin
- Species Reactivity
- Human, Monkey
- Clone
- 264RAD
- Applications
- ELISA, FC, Inhib, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The αVβ6 integrin antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Flow Cytometry, Inhibition, Functional Assay.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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See other products for "Clone 264RAD"
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1063 | Recombinant Anti-αvβ6 integrin Antibody | WB, IHC, FuncS | IgG |
| HPAB-0216LY | Human Anti-αVβ6 integrin Recombinant Antibody (clone sc 265RAD/ADY) | ELISA, FC | Human IgG1 |
| HPAB-0217LY | Human Anti-αVβ6 integrin Recombinant Antibody (clone sc 188SDM) | ELISA, FC | Human IgG1 |
| HPAB-0218LY | Human Anti-αVβ6 integrin Recombinant Antibody (clone sc 133TMT) | ELISA, FC | Human IgG1 |
| HPAB-0220LY | Human Anti-αVβ6 integrin Recombinant Antibody (clone sc 49) | ELISA, FC | Human IgG1 |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1063-S(P) | Recombinant Anti-αvβ6 integrin Antibody scFv Fragment | Neut, WB, FuncS | scFv |
| MHH-1063-S(P) | Recombinant Human Anti-αvβ6 integrin Antibody scFv Fragment | FC, ELISA, FuncS | scFv |
| HPAB-0215LY-S(P) | Human Anti-αVβ6 integrin Recombinant Antibody (clone 264RAD); scfv Fragment | ELISA, FC | Human scfv |
| HPAB-0316LY-S(P) | Human Anti-αVβ6 integrin Recombinant Antibody; scFv Fragment (HPAB-0316LY-S(P)) | Block, FC, IHC | Humanized scfv |
| HPAB-0317LY-S(P) | Human Anti-αVβ6 integrin Recombinant Antibody; scFv Fragment (HPAB-0317LY-S(P)) | Block, FC, IHC | Humanized scfv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-1063-F(E) | Recombinant Human Anti-αvβ6 integrin Antibody Fab Fragment | IF, FuncS | Fab |
| HPAB-0215LY-F(E) | Human Anti-αVβ6 integrin Recombinant Antibody (clone 264RAD); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-0216LY-F(E) | Human Anti-αVβ6 integrin Recombinant Antibody (clone sc 265RAD/ADY); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-0217LY-F(E) | Human Anti-αVβ6 integrin Recombinant Antibody (clone sc 188SDM); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-0218LY-F(E) | Human Anti-αVβ6 integrin Recombinant Antibody (clone sc 133TMT); Fab Fragment | ELISA, FC | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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