Mouse Anti-V5-Tag Recombinant Antibody (clone 9E8B7) (CAT#: ZG-0276U)
Mouse anti-V5-Tag antibody (clone 9E8B7) displayed high specificity against the V5-Tag.
Specific Inquiry
WB
Figure 1 Mouse Anti-V5-Tag Antibody (ZG-0276U) in WB
Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1: 2000 Lane 1: Recombinant V5-tagged fusion protein at 25ng
Lane 2: Recombinant V5-tagged fusion protein at 12.5ng
Lane 3: Recombinant V5-tagged fusion protein at 6.25ng
Lane 4: Recombinant V5-tagged fusion protein at 3.125ng
Lane 5: Recombinant V5-tagged fusion protein at 1.56ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 32 kd
Observed band size: 32 kd
Exposure time: 5min
IF
Figure 2 Mouse Anti-V5-Tag Antibody (ZG-0276U) in IF
Immunofluorescence staining of transfected HEK293F cells with ZG-0276U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L).
IP
Figure 3 Mouse Anti-V5-Tag Antibody (ZG-0276U) in IP
Immunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate Lane 1: Mouse control IgG2a in Transfected HEK293 cells whole cell lysate
Lane 2: ZG-0276U (2.5µl) + Transfected HEK293F cells whole cell lysate (500µg)
Lane 3: Transfected HEK293F cells whole cell lysate (20µg)
For western blotting, the blot was detected with ZG-0276U at 1:3000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
Specifications
- Immunogen
- GKPIPNPLLGLDST synthetic peptide conjugate to KLH
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- V5-Tag
- Species Reactivity
- All
- Clone
- 9E8B7
- Applications
- ELISA, WB, IF, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Immunoprecipitation.
Target
- Alternative Names
- V5-Tag
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0275U | Mouse Anti-V5-Tag Recombinant Antibody (clone 8A7A8) | ELISA, WB, IF, FC, IP | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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