Rabbit Anti-VASP Recombinant Antibody (clone R04-2B0)
CAT#: VS3-FY1932
This anti-VASP antibody detected positive in western blot analysis of VASP in Hela lysates. The recombinant monoclonal antibody with high affinity in binding to human VASP. It is suitable for use in a variety of assays, including WB, IP. This R04-2B0 antibody is validated for western blot analysis of VASP in Hela lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5724/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4612/if_selected.jpg
Normal Tissue
Figure 3 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5724/20155_B_8_4_val_selected.jpg
Normal Tissue
Figure 4 Small intestine.
High expression in small intestine
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4612/15247_A_4_2_selected.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5724/20155_A_7_5.jpg
Normal Tissue
Figure 6 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4612/15247_A_5_5.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5724/20155_A_9_8.jpg
Normal Tissue
Figure 8 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4612/15247_B_7_5.jpg
Normal Tissue
Figure 9 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5724/20155_A_7_3.jpg
Normal Tissue
Figure 10 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4612/15247_A_7_4.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (HMC-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125753-VASP
❮
❯
WB
Figure 1 Anti-VASP Antibody (VS3-FY1932) in WB.
The VASP expression in Hela lysates were detected using the anti-VASP by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human VASP
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human VASP
- Species Reactivity
- Human
- Clone
- R04-2B0
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 40 kDa, while the observed molecular weight stands at 46 kDa.
- Related Disease
- Thrombosis and Osteogenesis Imperfecta, Type Xvi
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Vasodilator Stimulated Phosphoprotein; Vasodilator-Stimulated Phosphoprotein
- Gene ID
- 7408
- UniProt ID
- P50552
- Sequence Similarities
- Belongs to the Ena/VASP family.
- Cellular Localization
- Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets.
The preferred site for PKA is Ser-157, the preferred site for PKG/PRKG1, Ser-239.
In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition.
Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239.
In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA.
In response to thrombin, phosphorylated by both PKC and ROCK1.
Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239.
Phosphorylation at Ser-157 by PKA is required for localization to the tight junctions in epithelial cells.
Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation.
Phosphorylation at Ser-322 by AMPK also alters actin filament binding.
- Protein Refseq
- XP_005259256.1; XP_005259257.1; XP_016882689.1; XP_054177916.1; XP_054177917.1
- Function
- Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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