Rabbit Anti-VASP Recombinant Antibody (clone R04-2B0) (CAT#: VS3-FY1932)
This anti-VASP antibody detected positive in western blot analysis of VASP in Hela lysates. The recombinant monoclonal antibody with high affinity in binding to human VASP. It is suitable for use in a variety of assays, including WB, IP. This R04-2B0 antibody is validated for western blot analysis of VASP in Hela lysates.
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WB
Figure 1 Anti-VASP Antibody (VS3-FY1932) in WB.
The VASP expression in Hela lysates were detected using the anti-VASP by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human VASP
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human VASP
- Species Reactivity
- Human
- Clone
- R04-2B0
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 40 kDa, while the observed molecular weight stands at 46 kDa.
- Related Disease
- Thrombosis and Osteogenesis Imperfecta, Type Xvi
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Vasodilator Stimulated Phosphoprotein; Vasodilator-Stimulated Phosphoprotein
- Gene ID
- 7408
- UniProt ID
- P50552
- Sequence Similarities
- Belongs to the Ena/VASP family.
- Cellular Localization
- Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets.
The preferred site for PKA is Ser-157, the preferred site for PKG/PRKG1, Ser-239.
In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition.
Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239.
In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA.
In response to thrombin, phosphorylated by both PKC and ROCK1.
Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239.
Phosphorylation at Ser-157 by PKA is required for localization to the tight junctions in epithelial cells.
Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation.
Phosphorylation at Ser-322 by AMPK also alters actin filament binding.
- Protein Refseq
- XP_005259256.1; XP_005259257.1; XP_016882689.1; XP_054177916.1; XP_054177917.1
- Function
- Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3824 | Rabbit Anti-VASP Recombinant Antibody (clone DS3824AB) | IHC-P, WB | Rabbit IgG |
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