Anti-VCAM-1 Immunohistochemistry Kit

Name: Anti-VCAM-1 Immunohistochemistry Kit
SKU: VS-0325-XY2463
Rating: 5 (1 reviews)

CAT#: VS-0325-XY2463

Designed for VCAM-1 detection, this IHC kit provides all necessary components, including an optimized antibody, for efficient staining. It is compatible with both paraffin-embedded and frozen tissues, allowing flexible sample handling. Valued for its consistent performance, the kit is suitable for studying immune cell adhesion and inflammation-related diseases.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cell junctions.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69867/1706_F12_18_cr57d839e75f94b_selected.jpg

Normal Tissue Figure 2 Kidney

Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_8_5.jpg

Normal Tissue Figure 3 Testis

Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_6_6.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_8_8.jpg

Normal Tissue Figure 5 High expression in spleen

Cells in red pulp Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_B_7_4.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (HHSteC, HSkMC)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162692-VCAM1

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Specifications

Application
IHC

Size
50 Tests

Species Reactivity
Human

Target
VCAM-1

Primary Antibody
Mouse Anti-VCAM-1 Monoclonal Antibody

Secondary Antibody
Goat anti-Mouse Antibody, HRP

Sample Type
FFPE tissue; Frozen section tissue

Kit Storage
All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.

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Related Signaling Pathways

TNF Signaling Pathway

Related Diseases

AGE-RAGE Signaling Pathway in Diabetic Complications

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Datasheet

Datasheet - VS-0325-XY2463.pdf
IHC Kits-FFPE Protocol.pdf
IHC Kits-FS Protocol.pdf

MSDS

MSDS - VS-0325-XY2463.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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CAT	Product Name	Application	Type
HPAB-0860-CN-F(E)	Human Anti-VCAM1 Recombinant Antibody (clone 7H); Fab Fragment	ELISA, Neut	Human Fab
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