Human Anti-VCAM1 Recombinant Antibody (clone ch2A2) (CAT#: FAMAB-0009JF)
Recombinant chimeric monoclonal antibody specially binds to pig VCAM1. This chimeric (mouse/human) anti-VCAM1 antibody (ch2A2) can be potential therapeutic use in xenotransplantation.
Specific Inquiry
WB
Figure 1 Western blot assay of VCAM-1 and β-actin.
P < 0.05 compared to the control group (two-sided paired t-test).
Zhan, Q., Yue, W., & Shaoshan, H. (2011). The inhibitory effect of photodynamic therapy and of an anti-VCAM-1 monoclonal antibody on the in vivo growth of C6 glioma xenografts. Brazilian Journal of Medical and Biological Research, 44(5), 489-490.
IHC
Figure 2 Immunohistochemistry for vascular cell adhesion molecule-1 (VCAM-1).
The arrows show the VCAM-1-positive cells.
Zhan, Q., Yue, W., & Shaoshan, H. (2011). The inhibitory effect of photodynamic therapy and of an anti-VCAM-1 monoclonal antibody on the in vivo growth of C6 glioma xenografts. Brazilian Journal of Medical and Biological Research, 44(5), 489-490.
ELISA
Figure 3 Kinetics of anti-proteinase 3 (PR3) antibody-induced VCAM-1 expression on human endothelial cells.
Cyto-ELISA with unfixed cells.
MAYET, W. J., Schwarting, A., Orth, T., Duchmann, R., & Meyer zum Büschenfelde, K. H. (1996). Antibodies to proteinase 3 mediate expression of vascular cell adhesion molecule‐1 (VCAM‐1). Clinical & Experimental Immunology, 103(2), 259-267.
IF
Figure 4 Detection of increased expression of VCAM-1 in the membrane of human endothelial cells.
Unfixed HEC preincubated for 8 h with purified anti-RNP antibodies (control).
MAYET, W. J., Schwarting, A., Orth, T., Duchmann, R., & Meyer zum Büschenfelde, K. H. (1996). Antibodies to proteinase 3 mediate expression of vascular cell adhesion molecule‐1 (VCAM‐1). Clinical & Experimental Immunology, 103(2), 259-267.
FC
Figure 5 Flow cytometric analysis of HAECs using antibody VCAM-1.
Relative cell numbers (vertical) and fluorescence intensity on log10 scale (horizontal) are indicated.
Tsao, P. S., Buitrago, R., Chan, J. R., & Cooke, J. P. (1996). Fluid flow inhibits endothelial adhesiveness: nitric oxide and transcriptional regulation of VCAM-1. Circulation, 94(7), 1682-1689.
Specifications
- Immunogen
- Secreted form of pVCAM
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- Human IgG4
- Specificity
- Pig VCAM1
- Species Reactivity
- Porcine
- Clone
- ch2A2HuG4
- Applications
- WB, ELISA, FC, IHC, IF
- Related Disease
- Diseases that need xenotransplantation
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The VCAM1 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry, Immunofluorescence.
Target
- Alternative Names
- VCAM1; vascular cell adhesion molecule1
- Gene ID
- 7412
- UniProt ID
- P19320
- Function
- Blocking
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-061 | Recombinant Anti-Human VCAM1 VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B067(A) | Recombinant Anti-human VCAM-1 Intrabody [(D-Arg)9] | FACS, ELISA, IF, FuncS | scFv-(D-Arg)9 |
| IAB-B067(G) | Recombinant Anti-human VCAM-1 Intrabody [+36 GFP] | FACS, ICC, FuncS | scFv-(+36GFP) |
| IAB-B067(T) | Recombinant Anti-human VCAM-1 Intrabody [Tat] | PCA, WB, FuncS | scFv-Tat |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-249CQ | Anti-Human VCAM1 Recombinant Antibody (H6) | ELISA | Human antibody |
| TAB-249CQ-S(P) | Anti-Human VCAM1 Recombinant Antibody scFv Fragment (H6) | ELISA, FC, Neut | Human antibody |
| TAB-249CQ-F(E) | Anti-Human VCAM1 Recombinant Antibody Fab Fragment (H6) | ELISA, FC, Neut | Human antibody |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-250CQ | Human Anti-VCAM1 Recombinant Antibody (TAB-250CQ) | ELISA, FC, WB, IHC | Humanized IgG |
| TAB-251CQ | Human Anti-VCAM1 Recombinant Antibody (TAB-251CQ) | ELISA, FC, WB, IHC | Humanized IgG |
| TAB-250CQ-S(P) | Human Anti-VCAM1 Recombinant Antibody; scFv Fragment (TAB-250CQ-S(P)) | ELISA, FC, WB, IHC | Humanized scFv |
| TAB-251CQ-S(P) | Human Anti-VCAM1 Recombinant Antibody; scFv Fragment (TAB-251CQ-S(P)) | ELISA, FC, WB, IHC | Humanized scFv |
| TAB-250CQ-F(E) | Human Anti-VCAM1 Recombinant Antibody; Fab Fragment (TAB-250CQ-F(E)) | ELISA, FC, WB, IHC | Humanized Fab |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2248CQ | Rat Anti-Vcam1 Recombinant Antibody (VMC209) | FC, IP, Block, IHC | Rat IgG2a, κ |
| NEUT-2249CQ | Rat Anti-Vcam1 Recombinant Antibody (clone M/K-2.7) | Block, IF | Rat IgG1 |
| NEUT-2251CQ | Mouse Anti-VCAM1 Recombinant Antibody (clone B-K9) | Block, FuncS, FC | Mouse IgG1 |
| NEUT-2252CQ | Mouse Anti-VCAM1 Recombinant Antibody (clone M/K-2), PE | FC, ICC, IF, Block, IP | Mouse IgG1, κ |
| NEUT-2254CQ | Mouse Anti-VCAM1 Recombinant Antibody (clone BBIG-V1) | Block, WB, IP, ICC | Mouse IgG1 |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2250CQ | Rat Anti-Vcam1 Recombinant Antibody (clone mAb0829) | Neut, FC, IHC, IHC-Fr, IP, CyTOF | Rat IgG1 |
| NEUT-2253CQ | Mouse Anti-VCAM1 Recombinant Antibody (clone 1.G11B1) | WB, Neut, ELISA, FC, IHC, IHC-Fr, IP, ICC | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3829 | Rabbit Anti-VCAM1 Recombinant Antibody (clone DS3829AB) | WB, ICC, IP | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0218-YC-S(P) | Human Anti-VCAM1 Recombinant Antibody (clone HS-1); scFv Fragment | ELISA, Neut | Rabbit scFv |
| HPAB-0860-CN-S(P) | Human Anti-VCAM1 Recombinant Antibody (clone 7H); scFv Fragment | ELISA, Neut | Human scFv |
| HPAB-AP205-YC-S(P) | Human Anti-VCAM1 Recombinant Antibody; scFv Fragment (HPAB-AP205-YC-S(P)) | IHC, DB | Human scFv |
| HPAB-AP206-YC-S(P) | Human Anti-VCAM1 Recombinant Antibody; scFv Fragment (HPAB-AP206-YC-S(P)) | IHC, DB | Human scFv |
| FAMAB-0009JF-S(P) | Mouse Anti-VCAM1 Recombinant Antibody (clone ch2A2); scFv Fragment | FC, ELISA, FuncS | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0218-YC-F(E) | Human Anti-VCAM1 Recombinant Antibody (clone HS-1); Fab Fragment | ELISA, Neut | Human Fab |
| HPAB-0860-CN-F(E) | Human Anti-VCAM1 Recombinant Antibody (clone 7H); Fab Fragment | ELISA, Neut | Human Fab |
| HPAB-AP205-YC-F(E) | Human Anti-VCAM1 Recombinant Antibody; Fab Fragment (HPAB-AP205-YC-F(E)) | IHC, DB | Human Fab |
| HPAB-AP206-YC-F(E) | Human Anti-VCAM1 Recombinant Antibody; Fab Fragment (HPAB-AP206-YC-F(E)) | IHC, DB | Human Fab |
| HPAB-AP207-YC-F(E) | Human Anti-VCAM1 Recombinant Antibody; Fab Fragment (HPAB-AP207-YC-F(E)) | IHC, DB | Human Fab |
Neuroscience Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-100CN | Human Anti-VCAM1 Recombinant Antibody (clone 7C) | ELISA | Human IgG |
| NS-101CN | Human Anti-VCAM1 Recombinant Antibody (clone 4B) | ELISA | Human IgG |
| NS-100CN-F(E) | Human Anti-VCAM1 Recombinant Antibody (clone 7C); Fab Fragment | ELISA | Human Fab |
| NS-101CN-F(E) | Human Anti-VCAM1 Recombinant Antibody (clone 4B); Fab Fragment | ELISA | Human Fab |
| NS-100CN-S(P) | Human Anti-VCAM1 Recombinant Antibody (clone 7C); scFv Fragment | ELISA | Human scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0860-CN | Human Anti-VCAM1 Recombinant Antibody (clone 7H) | ELISA, Neut | Human IgG |
| MRO-1574-CN | Rabbit Anti-VCAM1 Recombinant Antibody (clone CBACN-576) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0498F | Mouse Anti-VCAM1 Recombinant Antibody (ZG-0498F) | WB, IHC, ELISA | Mouse IgG |
| VS3-QX1231 | Mouse Anti-VCAM1 Recombinant Antibody (clone 6G9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY1538 | Recombinant Rabbit Anti-Vcam1 Antibody (clone R07-6E9) | WB, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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