CytoStream™ Mouse Anti-VCAM1 Recombinant Antibody (VS-0225-XY231)
CAT#: VS-0225-XY231
This mouse-derived antibody targets Vascular Cell Adhesion Molecule 1 (VCAM-1), a crucial mediator in immune cell trafficking and inflammation. It exhibits strong reactivity to human samples, making it highly suitable for human-based research. The antibody is compatible with a variety of applications, including Flow Cytometry (FC), Immunohistochemistry (IHC), Blocking, ELISA, and Immunoprecipitation (IP), offering a versatile tool for detailed studies of VCAM-1's role in cellular interactions and disease processes.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69867/1706_F12_18_cr57d839e75f94b_selected.jpg
Normal Tissue
Figure 2 Kidney
Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_8_8.jpg
Normal Tissue
Figure 5 High expression in spleen
Cells in red pulp Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_B_7_4.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HHSteC, HSkMC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162692-VCAM1
❮
❯
Specifications
- Immunogen
- Human VCAM1 Recombinant Protein
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- VCAM1
- Species Reactivity
- Human
- Applications
- FC, IHC, Block, ELISA, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- Sodium azide
- Storage
- Store at 4°C for short-term storage and at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Target
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Related Signaling Pathways
TNF Signaling Pathway
Related Diseases
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1467z | Mouse Anti-VCAM1 Recombinant Antibody (clone 23B9) | ELISA, FC, IHC, FuncS | Mouse IgG1 |
| HPAB-0218-YC | Human Anti-VCAM1 Recombinant Antibody (clone HS-1) | ELISA, Neut | Chimeric (rabbit/human) IgG |
| MRO-1574-CN | Rabbit Anti-VCAM1 Recombinant Antibody (clone CBACN-576) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-QX1231 | Mouse Anti-VCAM1 Recombinant Antibody (clone 6G9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-CJ745 | Rabbit Anti-VCAM1 Recombinant Antibody (VS3-CJ745) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-061 | Recombinant Anti-Human VCAM1 VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B067(A) | Recombinant Anti-human VCAM-1 Intrabody [(D-Arg)9] | FACS, ELISA, IF, FuncS | scFv-(D-Arg)9 |
| IAB-B067(G) | Recombinant Anti-human VCAM-1 Intrabody [+36 GFP] | FACS, ICC, FuncS | scFv-(+36GFP) |
| IAB-B067(T) | Recombinant Anti-human VCAM-1 Intrabody [Tat] | PCA, WB, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-249CQ | Anti-Human VCAM1 Recombinant Antibody (H6) | ELISA | Human antibody |
| TAB-249CQ-S(P) | Anti-Human VCAM1 Recombinant Antibody scFv Fragment (H6) | ELISA, FC, Neut | Human antibody |
| TAB-249CQ-F(E) | Anti-Human VCAM1 Recombinant Antibody Fab Fragment (H6) | ELISA, FC, Neut | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-250CQ | Human Anti-VCAM1 Recombinant Antibody (TAB-250CQ) | ELISA, FC, WB, IHC | Humanized IgG |
| TAB-251CQ | Human Anti-VCAM1 Recombinant Antibody (TAB-251CQ) | ELISA, FC, WB, IHC | Humanized IgG |
| TAB-250CQ-S(P) | Human Anti-VCAM1 Recombinant Antibody; scFv Fragment (TAB-250CQ-S(P)) | ELISA, FC, WB, IHC | Humanized scFv |
| TAB-251CQ-S(P) | Human Anti-VCAM1 Recombinant Antibody; scFv Fragment (TAB-251CQ-S(P)) | ELISA, FC, WB, IHC | Humanized scFv |
| TAB-250CQ-F(E) | Human Anti-VCAM1 Recombinant Antibody; Fab Fragment (TAB-250CQ-F(E)) | ELISA, FC, WB, IHC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2248CQ | Rat Anti-Vcam1 Recombinant Antibody (VMC209) | FC, IP, Block, IHC | Rat IgG2a, κ |
| NEUT-2249CQ | Rat Anti-Vcam1 Recombinant Antibody (clone M/K-2.7) | Block, IF | Rat IgG1 |
| NEUT-2251CQ | Mouse Anti-VCAM1 Recombinant Antibody (clone B-K9) | Block, FuncS, FC | Mouse IgG1 |
| NEUT-2252CQ | Mouse Anti-VCAM1 Recombinant Antibody (NEUT-2252CQ) | FC, ICC, IF, Block, IP | Mouse IgG1, κ |
| NEUT-2254CQ | Mouse Anti-VCAM1 Recombinant Antibody (NEUT-2254CQ) | Block, WB, IP, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2250CQ | Rat Anti-Vcam1 Recombinant Antibody (NEUT-2250CQ) | Neut, FC, IHC, IHC-Fr, IP, CyTOF | Rat IgG1 |
| NEUT-2253CQ | Mouse Anti-VCAM1 Recombinant Antibody (clone 1.G11B1) | WB, Neut, ELISA, FC, IHC, IHC-Fr, IP, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3829 | Rabbit Anti-VCAM1 Recombinant Antibody (clone DS3829AB) | WB, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0218-YC-S(P) | Human Anti-VCAM1 Recombinant Antibody (clone HS-1); scFv Fragment | ELISA, Neut | Rabbit scFv |
| HPAB-0860-CN-S(P) | Human Anti-VCAM1 Recombinant Antibody (clone 7H); scFv Fragment | ELISA, Neut | Human scFv |
| HPAB-AP205-YC-S(P) | Human Anti-VCAM1 Recombinant Antibody; scFv Fragment (HPAB-AP205-YC-S(P)) | IHC, DB | Human scFv |
| HPAB-AP206-YC-S(P) | Human Anti-VCAM1 Recombinant Antibody; scFv Fragment (HPAB-AP206-YC-S(P)) | IHC, DB | Human scFv |
| FAMAB-0009JF-S(P) | Mouse Anti-VCAM1 Recombinant Antibody (clone ch2A2); scFv Fragment | FC, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-100CN | Human Anti-VCAM1 Recombinant Antibody (clone 7C) | ELISA | Human IgG |
| NS-101CN | Human Anti-VCAM1 Recombinant Antibody (clone 4B) | ELISA | Human IgG |
| NS-100CN-F(E) | Human Anti-VCAM1 Recombinant Antibody (clone 7C); Fab Fragment | ELISA | Human Fab |
| NS-101CN-F(E) | Human Anti-VCAM1 Recombinant Antibody (clone 4B); Fab Fragment | ELISA | Human Fab |
| NS-100CN-S(P) | Human Anti-VCAM1 Recombinant Antibody (clone 7C); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0860-CN-F(E) | Human Anti-VCAM1 Recombinant Antibody (clone 7H); Fab Fragment | ELISA, Neut | Human Fab |
| HPAB-AP205-YC-F(E) | Human Anti-VCAM1 Recombinant Antibody; Fab Fragment (HPAB-AP205-YC-F(E)) | IHC, DB | Human Fab |
| HPAB-AP206-YC-F(E) | Human Anti-VCAM1 Recombinant Antibody; Fab Fragment (HPAB-AP206-YC-F(E)) | IHC, DB | Human Fab |
| HPAB-AP207-YC-F(E) | Human Anti-VCAM1 Recombinant Antibody; Fab Fragment (HPAB-AP207-YC-F(E)) | IHC, DB | Human Fab |
| FAMAB-0009JF-F(E) | Human Anti-VCAM1 Recombinant Antibody (clone ch2A2); Fab Fragment | FC, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY272 | AbPlus™ Anti-VCAM1 Magnetic Beads (1.G11B1) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY210 | Human Anti-VCAM1 (clone H6) scFv-Fc Chimera | ELISA, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC605 | Recombinant Anti-VCAM1 Vesicular Antibody, EV Displayed (VS-0425-YC605) | ELISA, FC, Neut, Cell-uptake |
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