Human Anti-VWF Recombinant Antibody (clone h82D6A3) (CAT#: FAMAB-0297YC)
Provided is humanized antibody, h82D6A3, by variable domain resurfacing and grafting on the constant regions of a human IgG4. The h82D6A3 still binding with high affinity to VWF and retained the inhibitory properties of the murine 82D6A3.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Inhib
Figure 1 Inhibition of VWF-binding to human collagen type III.
Before addition to collagen coated wells, a constant concentration of purified human VWF (250 ng/ml) was pre-incubated with different concentrations of purified h82D6A3 or m82D6A3. Bound VWF was detected with anti-VWF-HRP antibodies. The absorbance of VWF-binding in the absence of 82D6A3 was arbitrarily set as 0% inhibition.
Staelens, S., Desmet, J., Ngo, T. H., Vauterin, S., Pareyn, I., Barbeaux, P., ... & Vanhoorelbeke, K. (2006). Humanization by variable domain resurfacing and grafting on a human IgG4, using a new approach for determination of non-human like surface accessible framework residues based on homology modelling of variable domains. Molecular immunology, 43(8), 1243-1257.
Specifications
- Immunogen
- Human VWF
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG4
- Specificity
- Human VWF
- Species Reactivity
- Human
- Clone
- h82D6A3
- Applications
- Inhib
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Inhibion. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibodies
Fab Antibodies
ScFv Antibodies
Mouse Antibodies
Humanized Antibodies
Chimeric Antibodies
Rabbit Monoclonal Antibodies
Single Domain Antibodies
ScFv-Fc Chimeras
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-132 | Mouse Anti-VWF Recombinant Antibody (clone 82D6A3) | WB, IF, FuncS | Mouse IgG |
| PABW-132 | Mouse Anti-VWF Recombinant Antibody (clone NMC-4) | Block, Inhib, WB, DB | Mouse IgG |
| PABC-133 | Mouse Anti-vWF Recombinant Antibody (clone RU5) | ELISA, WB, IHC, IF, Inhib, FuncS | Mouse IgG |
| FAMAB-1291CQ | Human Anti-VWF Recombinant Antibody (clone F8/44/20) | WB, ELISA, IHC | Human IgG1 |
| VS-0723-WK146 | Human Anti-VWF Recombinant Antibody (VS-0723-WK146) | FC | Chimeric (rabbit/human) IgG1 |
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