Human Anti-VWF Recombinant Antibody (clone h82D6A3)
CAT#: FAMAB-0297YC
Provided is humanized antibody, h82D6A3, by variable domain resurfacing and grafting on the constant regions of a human IgG4. The h82D6A3 still binding with high affinity to VWF and retained the inhibitory properties of the murine 82D6A3.
Published Data
Gene Expression
Inhib
Figure 1 Inhibition of VWF-binding to human collagen type III.
Before addition to collagen coated wells, a constant concentration of purified human VWF (250 ng/ml) was pre-incubated with different concentrations of purified h82D6A3 or m82D6A3. Bound VWF was detected with anti-VWF-HRP antibodies. The absorbance of VWF-binding in the absence of 82D6A3 was arbitrarily set as 0% inhibition.
Staelens, S., Desmet, J., Ngo, T. H., Vauterin, S., Pareyn, I., Barbeaux, P., ... & Vanhoorelbeke, K. (2006). Humanization by variable domain resurfacing and grafting on a human IgG4, using a new approach for determination of non-human like surface accessible framework residues based on homology modelling of variable domains. Molecular immunology, 43(8), 1243-1257.
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Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1694/4967_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1694/4967_A_9_3.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (HUVEC TERT2)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (HUVEC TERT2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110799-VWF
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Specifications
- Immunogen
- Human VWF
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG4
- Specificity
- Human VWF
- Species Reactivity
- Human
- Clone
- h82D6A3
- Applications
- Inhib
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Inhibion. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone h82D6A3"
- CAT
- Product Name
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Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-132 | Mouse Anti-VWF Recombinant Antibody (clone 82D6A3) | WB, IF, FuncS | Mouse IgG |
| PABW-132 | Mouse Anti-VWF Recombinant Antibody (clone NMC-4) | Block, Inhib, WB, DB | Mouse IgG |
| PABC-133 | Mouse Anti-vWF Recombinant Antibody (clone RU5) | ELISA, WB, IHC, IF, Inhib, FuncS | Mouse IgG |
| MOB-177CQ | Mouse Anti-VWF Recombinant Antibody (clone YC161) | WB, IP, IF, IHC-P | Mouse IgG1, κ |
| ZG-507C | Mouse Anti-VWF Recombinant Antibody (clone RU8) | ELISA, immunopurification of vWF | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-132 | Mouse Anti-VWF Recombinant Antibody (clone 82D6A3); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| PFBW-132 | Mouse Anti-VWF Recombinant Antibody (clone NMC-4); Fab Fragment | Block | Mouse Fab |
| HPAB-0343-WJ-F(E) | Mouse Anti-VWF Recombinant Antibody; Fab Fragment (HPAB-0343-WJ-F(E)) | ELISA, WB | Mouse Fab |
| HPAB-M0250-YC-F(E) | Mouse Anti-VWF Recombinant Antibody (clone SZ123); Fab Fragment | Inhib, FuncS | Mouse Fab |
| HPAB-M0251-YC-F(E) | Human Anti-VWF Recombinant Antibody (clone MHC-SZ123); Fab Fragment | Inhib, FuncS | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-132 | Mouse Anti-VWF Recombinant Antibody (clone 82D6A3); scFv Fragment | WB, IF, FuncS | Mouse scFv |
| FAMAB-0297-YC-S(P) | Human Anti-VWF Recombinant Antibody (clone h82D6A3); scFv Fragment | ELISA, WB | Humanized scFv |
| FAMAB-0298-YC-S(P) | Mouse Anti-VWF Recombinant Antibody (clone m-82D6A3); scFv Fragment | ELISA, WB | Mouse scFv |
| HPAB-M0250-YC-S(P) | Mouse Anti-VWF Recombinant Antibody (clone SZ123); scFv Fragment | Inhib, FuncS | Mouse scFv |
| HPAB-M0251-YC-S(P) | Human Anti-VWF Recombinant Antibody (clone MHC-SZ123); scFv Fragment | Inhib, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-362CQ | Anti-Human VWF Recombinant Antibody (AJvW-2) | ELISA, BL | |
| TAB-362CQ-S(P) | Anti-Human VWF Recombinant Antibody scFv Fragment (AJvW-2) | ELISA, BL | |
| TAB-363CQ-S(P) | Mouse Anti-VWF Recombinant Antibody; scFv Fragment (TAB-363CQ-S(P)) | ELISA, WB, Inhib | Mouse scFv |
| TAB-362CQ-F(E) | Anti-Human VWF Recombinant Antibody Fab Fragment (AJvW-2) | ELISA, BL | |
| TAB-363CQ-F(E) | Mouse Anti-VWF Recombinant Antibody; Fab Fragment (TAB-363CQ-F(E)) | ELISA, WB, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-364CQ | Human Anti-VWF Recombinant Antibody (TAB-364CQ) | Inhib | Humanized antibody |
| TAB-364CQ-S(P) | Human Anti-VWF Recombinant Antibody; scFv Fragment (TAB-364CQ-S(P)) | Inhib | Humanized scFv |
| TAB-364CQ-F(E) | Human Anti-VWF Recombinant Antibody; Fab Fragment (TAB-364CQ-F(E)) | Inhib | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-365CQ | Human Anti-VWF Recombinant Antibody (TAB-365CQ) | Inhib | Chimeric antibody (mouse/human) |
| TAB-365CQ-S(P) | Mouse Anti-VWF Recombinant Antibody; scFv Fragment (TAB-365CQ-S(P)) | Inhib | Mouse scFv |
| TAB-365CQ-F(E) | Human Anti-VWF Recombinant Antibody; Fab Fragment (TAB-365CQ-F(E)) | Inhib | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3860 | Rabbit Anti-VWF Recombinant Antibody (clone DS3860AB) | WB, ICC, IF | Rabbit IgG |
| MOR-0109-FY | Rabbit Anti-VWF Recombinant Antibody (clone AFY0080) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1628-FY | Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1628-FY) | ELISA | Llama VHH |
| HPAB-1629-FY | Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1629-FY) | ELISA | Llama VHH |
| HPAB-1630-FY | Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1630-FY) | Inhib, ELISA | Llama VHH |
| HPAB-1631-FY | Recombinant Llama Anti-VWF Single Domain Antibody (HPAB-1631-FY) | ELISA, SPR, FC | Llama VHH |
| HPAB-1632-FY | Recombinant Llama Anti-VWF Single Domain Antibody (HPAB-1632-FY) | Inhib | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY41 | Human Anti-vWF (clone AJvW-2) scFv-Fc Chimera | ELISA, Block | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2481 | Anti-VWF Immunohistochemistry Kit | IHC | |
| VS-0525-XY7888 | Anti-Mouse VWF Immunohistochemistry Kit | IHC | |
| VS-0525-XY7887 | Anti-Human VWF Immunohistochemistry Kit | IHC |
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