CytoStream™ Rabbit Anti-WAS Recombinant Antibody (VS13-YC1226)
CAT#: VS13-YC1226
The CytoStream™ Rabbit Anti-WAS Recombinant Antibody is specific for human, mouse and rat WAS. WAS family proteins transduce signals affecting the actin cytoskeleton. Mutations cause Wiskott-Aldrich syndrome, characterized by immune dysregulation. The anti-WAS antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/1813_F8_24_cr59f33e96be102_selected.jpg
Normal Tissue
Figure 2 High expression in appendix
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_1_2_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_B_8_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4290/12454_A_6_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, MOLT-4, NB-4, RPMI-8226, THP-1, U-266/70, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015285-WAS
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Specifications
- Immunogen
- Recombinant human WASP.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat WAS
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IP, FC
- Conjugate
- NONE
- Related Disease
- Wiskott-Aldrich syndrome
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/40
Flow cytometry (FC): 1/50-1/100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "WAS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3677z | Mouse Anti-WAS Recombinant Antibody (clone 22F10) | WB, ELISA, FC, IHC | Mouse IgG2a |
| MOB-2231CT | Recombinant Mouse anti-Human WAS Monoclonal antibody (EML2139) | WB | |
| HPAB-0815-CN | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1) | ELISA, IHC | Rabbit IgG |
| VS3-WK20 | Mouse Anti-WAS Recombinant Antibody (clone E10-4B) | WB, IHC, FC | Mouse IgG2a |
| VS7-0425-WR1122 | Mouse Anti-WAS Recombinant Antibody (clone 7B10E4) | IHC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B076(A) | Recombinant Anti-mouse Was Intrabody [(D-Arg)9] | ELISA, WB, ICC, FuncS | scFv-(D-Arg)9 |
| IAB-B076(G) | Recombinant Anti-mouse Was Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B076(T) | Recombinant Anti-mouse Was Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0627MZ | Chicken Anti-WAS Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3863 | Rabbit Anti-WAS Recombinant Antibody (clone DS3863AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0815-CN-S(P) | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1); scFv Fragment | ELISA, IHC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0815-CN-F(E) | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1); Fab Fragment | ELISA, IHC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2484 | Anti-WAS Immunohistochemistry Kit | IHC | |
| VS-0525-XY7892 | Anti-Human WAS Immunohistochemistry Kit | IHC |
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