Mouse Anti-WT1 Recombinant Antibody (VS3-CJ752)
CAT#: VS3-CJ752
This product is a mouse antibody that recognizes human WT1.
Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence within human Wilms Tumor 1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human WT1
- Species Reactivity
- Human
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.2% BSA, PBS.
- Preservative
- 0.01% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:1000), Immunohistochemistry (1:100-1:300).
Target
- Alternative Names
- GUD; AWT1; WAGR; WT-1; WT33; NPHS4; WIT-2
- Gene ID
- 7490
- UniProt ID
- P19544
- Sequence Similarities
- Belongs to the EGR C2H2-type zinc-finger protein family.
- Cellular Localization
- Cytoplasm, Nucleus
- Protein Refseq
- NP_000369.3; NP_001185480.1; NP_001185481.1
- Function
- Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533).
Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363).
Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190).
Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801).
Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "WT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G065R | Anti-WT1 immunotoxin 6F-H2 (IgG)-RTA | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0344MZ | Recombinant Mouse Anti-Human Wilms Tumor 1 Antibody (clone 7G-H3) | IHC | Mouse antibody |
| MRO-1597-CN | Rabbit Anti-WT1 Recombinant Antibody (clone CBACN-584) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ753 | Rabbit Anti-WT1 Recombinant Antibody (VS3-CJ753) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-FY1556 | Recombinant Mouse Anti-WT1 Antibody (clone 7F9E8) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY1557 | Recombinant Mouse Anti-WT1 Antibody (clone 9D1-8B8-4B8) | IHC-P | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3886 | Rabbit Anti-WT1 Recombinant Antibody (clone DS3886AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY545 | Mouse Anti-NHP WT1 Recombinant Antibody (clone 7F9E8) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7945 | Anti-WT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7946 | Anti-Mouse WT1 Immunohistochemistry Kit | IHC |
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