Mouse Anti-XRCC4 Recombinant Antibody (VS3-FY2921)
CAT#: VS3-FY2921
This XRCC4 mouse monoclonal antibody is a recombinant monoclonal antibody that targets for human XRCC4 protein. It cross-reacts with mouse, rat species. The antibody can be utilized for detection of target protein by Immunoprecipitation (1/20), Western Blot (1/500-1/1000), Immunohistochemistry (1/50-1/100) and Immunocytochemistry (1/50-1/200).
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse and Rat XRCC4
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
- Conjugate
- Unconjugated, FITC
- Related Disease
- Short Stature, Microcephaly, And Endocrine Dysfunction and Microcephalic Primordial Dwarfism-Insulin Resistance Syndrome
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunocytochemistry: 1/50-1/200
Target
- Gene ID
- 7518
- UniProt ID
- Q13426
- Sequence Similarities
- Belongs to the XRCC4-XLF family. XRCC4 subfamily.
- Cellular Localization
- Nucleus
Chromosome
- Post Translation Modifications
- Ubiquitination at Lys197 and Lys296 (NX_Q13426 [NX_Q13426-1])
Modification sites at PhosphoSitePlus
- Protein Refseq
- XP_016865316.1; XP_016865317.1; XP_047273650.1; XP_047273651.1
- Function
- Acts as a scaffold protein that regulates recruitment of other proteins to DNA double-strand breaks (DSBs) (PubMed:15385968, 20852255, 26774286, 27437582).
Associates with NHEJ1/XLF to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired (PubMed:26100018, 27437582, 28500754, 21775435, 22287571, 21768349).
The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other.
Plays a key role in the NHEJ ligation step of the broken DNA during DSB repair via direct interaction with DNA ligase IV (LIG4): the LIG4-XRCC4 subcomplex reseals the DNA breaks after the gap filling is completed
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "XRCC4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0178C | Mouse Anti-XRCC4 Recombinant Antibody (clone 5C10) | WB, IHC-P, IF, IP | Mouse IgG |
| VS3-WK933 | Mouse Anti-XRCC4 Recombinant Antibody (VS3-WK933) | WB, IHC, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2499 | Anti-XRCC4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7966 | Anti-Human XRCC4 Immunohistochemistry Kit | IHC |
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