Mouse Anti-YWHAZ Recombinant Antibody (VS3-CJ1100) (CAT#: VS3-CJ1100)

• Immunogen
• Recombinant protein from human YWHAZ

• Host Species
• Mouse

• Type
• Mouse IgG2b, κ

• Specificity
• Human, Mouse, Rat YWHAZ

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, IF

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• PBS, pH 7.3.

• Preservative
• 0.09% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000), Immunofluorescence (1:25).

• Alternative Names
• HEL4; YWHAD; KCIP-1; HEL-S-3; POPCHAS; HEL-S-93; 14-3-3-zeta

• Gene ID
• 7534

• UniProt ID
• P63104

• Sequence Similarities
• Belongs to the 14-3-3 family.

• Cellular Localization
• Cytoplasm

• Post Translation Modifications
• The delta, brain-specific form differs from the zeta form in being phosphorylated (Probable). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria (PubMed:15071501, PubMed:15696159).
  Phosphorylation on Thr-232; inhibits binding of RAF1 (PubMed:9360956).
  Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion (PubMed:11956222, PubMed:12865427, PubMed:15883165, PubMed:16376338).
  Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53 (PubMed:11956222, PubMed:12865427, PubMed:15883165, PubMed:16376338).

• Protein Refseq
• NP_001129171.1; NP_001129172.1; NP_001129173.1

• Function
• Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation. In neurons, regulates spine maturation through the modulation of ARHGEF7 activity.

Isotype Control

• MOB-212YJL
• Mouse IgG2b kappa Isotype Control Antibody (Mopc21)

• MOB-203CQ
• Mouse IgG2b, kappa Isotype Control Antibody

Secondary Antibody

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS