Mouse Anti-ZAP70 Recombinant Antibody (ZG-0515F)
CAT#: ZG-0515F
This product is a mouse antibody that recognizes ZAP70.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-ZAP70 Recombinant Antibody (ZG-0515F) in WB.
Western blot detection of ZAP-70 in CEM and Jurkat cell lysates using ZAP-70 mouse mAb (1:1000 dilution) was negative in Ramos cell lysates. Predicted band size: 70KDa. Observed band size: 70KDa.
IP
Figure 2 Mouse Anti-ZAP70 Recombinant Antibody (ZG-0515F) in IP.
The ZAP-70 mouse mAb was used for immunoprecipitation analysis of Jurkat cell lysates (ZAP-70 positive expressing cell line) and Ramos cell lysates (ZAP-70 negative expressing cell line).
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Normal Tissue
Figure 1 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3134/8986_A_8_5.jpg
Normal Tissue
Figure 2 Bone marrow
Hematopoietic cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3134/8986_B_5_4.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3134/8986_A_7_8.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115085-ZAP70/subcellular
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Specifications
- Immunogen
- Recombinant human ZAP-70 protein fragments expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Antibody Isotype
- IgG
- Specificity
- Human ZAP70
- Species Reactivity
- Human
- Applications
- Western Blot
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000
Target
- Alternative Names
- 70 kDa zeta associated protein; 70 kDa zeta-associated protein; EC 2.7.10.2; FLJ17670; FLJ17679; Selective T cell defect; SRK; STD; Syk related tyrosine kinase; Syk-related tyrosine kinase; Truncated ZAP kinase; Tyrosine protein kinase ZAP70; Tyrosine-protein kinase ZAP-70; TZK; ZAP 70; ZAP- 70; ZAP70; ZAP70_HUMAN; Zeta chain associated protein kinase 70kD; Zeta chain associated protein kinase 70kDa; Zeta chain associated protein kinase 70kDa isoform 1; Zeta chain associated protein kinase 70kDa isoform 2; Zeta chain TCR associated protein kinase 70kD; Zeta chain TCR associated protein kinase 70kDa.
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Related Signaling Pathways
BCR Signaling Pathway
TCR Signaling Pathway
Related Diseases
Primary Immunodeficiency
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ZAP70"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2065z | Mouse Anti-ZAP70 Recombinant Antibody (clone 33A11) | FC, IF, IHC | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1794MZ | Recombinant Mouse Anti-Human ZAP70 Antibody (clone 22I50) | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
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| MOR-3911 | Rabbit Anti-ZAP70 Recombinant Antibody (clone DS3911AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MOR-4716 | Rabbit Anti-ZAP70 Recombinant Antibody (clone TH230DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1610-CN | Rabbit Anti-ZAP70 Recombinant Antibody (clone JU08-39) | WB, IF, IHC, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0514F | Mouse Anti-ZAP70 Recombinant Antibody (ZG-0514F) | WB, IHC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0179C | Mouse Anti-ZAP70 Recombinant Antibody (clone ABT-ZAP70) | IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1237 | CytoStream™ Rabbit Anti-ZAP70 Recombinant Antibody (VS13-YC1237) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7999 | Anti-ZAP70 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY8000 | Anti-Mouse ZAP70 Immunohistochemistry Kit | IHC |
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