Rabbit Anti-ZBTB33 Recombinant Antibody (clone R05-5J5)

CAT#: VS3-FY1752

This R05-5J5 recombinant monoclonal antibody is specifically engineered to detect human ZBTB33. It cross-reacts with rat. The binding specificity of the Kaiso antibody was determined using western blot analysis of Kaiso in MCF-7, C6 lysates.

Tested Data
Figure 1 Anti-ZBTB33 Antibody (VS3-FY1752) in WB.

Specifications

  • Immunogen
  • Synthetic peptide of human Kaiso
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Rat ZBTB33
  • Species Reactivity
  • Human, Rat
  • Clone
  • R05-5J5
  • MW
  • The calculated weight of the target protein is approximately 74 kDa, while the observed molecular weight stands at 90 kDa.
  • Related Disease
  • Cri-Du-Chat Syndrome

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000

Target

  • Alternative Names
  • Zinc Finger And BTB Domain Containing 33; ZBTB33
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Post Translation Modifications
  • Isopeptide bond
    Phosphoprotein
    Ubl conjugation
  • Function
  • Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

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Isotype Control

Secondary Antibody

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