Rabbit Anti-ZBTB33 Recombinant Antibody (clone R05-5J5) (CAT#: VS3-FY1752)
This R05-5J5 recombinant monoclonal antibody is specifically engineered to detect human ZBTB33. It cross-reacts with rat. The binding specificity of the Kaiso antibody was determined using western blot analysis of Kaiso in MCF-7, C6 lysates.
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WB
Figure 1 Anti-ZBTB33 Antibody (VS3-FY1752) in WB.
The Kaiso expression in MCF-7, C6 lysates were detected using the anti-Kaiso by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human Kaiso
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat ZBTB33
- Species Reactivity
- Human, Rat
- Clone
- R05-5J5
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 74 kDa, while the observed molecular weight stands at 90 kDa.
- Related Disease
- Cri-Du-Chat Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Zinc Finger And BTB Domain Containing 33; ZBTB33
- Gene ID
- 10009
- UniProt ID
- Q86T24
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Isopeptide bond
Phosphoprotein
Ubl conjugation
- Protein Refseq
- NP_001171671.1; NP_006768.1
- Function
- Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3636z | Mouse Anti-ZBTB33 Recombinant Antibody (clone 34G11) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-2152MZ | Recombinant Mouse Anti-Human ZBTB33 Antibody (clone 7G / 7G9) | ELISA, ICC/IF, IHC-P, IP, WB | Mouse antibody |
| VS13-YC1238 | Rabbit Anti-ZBTB33 Recombinant Antibody (VS13-YC1238) | WB | Rabbit IgG |
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