Rabbit Anti-ZFYVE9 Recombinant Antibody (clone R01-0A4) (CAT#: VS3-FY2083)
We conducted western blot analysis of SARA in lysates from HeLa, A549, and HL-60 cell lines utilizing the SARA antibody. The recombinant monoclonal antibody with high affinity in targeting human ZFYVE9. It is suitable for use in a variety of assays, including WB, IHC-P, IP. This R01-0A4 antibody is validated for western blot analysis of SARA in Hela, A549, HL-60 lysates.
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WB
Figure 1 Anti-ZFYVE9 Antibody (VS3-FY2083) in WB.
The SARA expression in Hela, A549, HL-60 lysates were detected using the anti-SARA by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human SARA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ZFYVE9
- Species Reactivity
- Human
- Clone
- R01-0A4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 156 kDa, while the observed molecular weight stands at 156 kDa.
- Related Disease
- Iga Glomerulonephritis and Nanophthalmos
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Zinc Finger FYVE-Type Containing 9; ZFYVE9
- Gene ID
- 9372
- UniProt ID
- O95405
- Cellular Localization
- Cytoplasm, Endosome, Membrane
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (O95405)
Modification sites at neXtProt (NX_O95405)
Glycosylation from GlyGen (O95405) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011540739.1; XP_047290630.1; XP_047290638.1; XP_054195648.1; XP_054195649.1
- Function
- Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.
Recommended Products
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Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY8026 | Anti-ZFYVE9 Immunohistochemistry Kit | IHC |
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For Research Use Only. Not For Clinical Use.
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