Rabbit Anti-ZFYVE9 Recombinant Antibody (clone R01-0A4)
CAT#: VS3-FY2083
We conducted western blot analysis of SARA in lysates from HeLa, A549, and HL-60 cell lines utilizing the SARA antibody. The recombinant monoclonal antibody with high affinity in targeting human ZFYVE9. It is suitable for use in a variety of assays, including WB, IHC-P, IP. This R01-0A4 antibody is validated for western blot analysis of SARA in Hela, A549, HL-60 lysates.
Tested Data
WB
Figure 1 Anti-ZFYVE9 Antibody (VS3-FY2083) in WB.
The SARA expression in Hela, A549, HL-60 lysates were detected using the anti-SARA by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human SARA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ZFYVE9
- Species Reactivity
- Human
- Clone
- R01-0A4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 156 kDa, while the observed molecular weight stands at 156 kDa.
- Related Disease
- Iga Glomerulonephritis and Nanophthalmos
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Zinc Finger FYVE-Type Containing 9; ZFYVE9
- Gene ID
- 9372
- UniProt ID
- O95405
- Cellular Localization
- Cytoplasm, Endosome, Membrane
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (O95405)
Modification sites at neXtProt (NX_O95405)
Glycosylation from GlyGen (O95405) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011540739.1; XP_047290630.1; XP_047290638.1; XP_054195648.1; XP_054195649.1
- Function
- Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ZFYVE9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY8026 | Anti-ZFYVE9 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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