Antibody-Oligonucleotide Conjugate Development Services

Creative Biolabs is a customized CRO company with in-depth knowledge of antibody-oligonucleotide conjugates (AOCs) research, providing you with excellent services in the design, development, and validation of AOC products.

Platform

Bioconjugates have revolutionized the field of life sciences by allowing for precise and targeted delivery of therapeutics. One of the most promising bioconjugates currently in development is antibody-oligonucleotide conjugates (AOCs). At Creative Biolabs, we are at the forefront of AOC development, offering comprehensive services for the design, synthesis, and characterization of these innovative compounds. Our team of experts is skilled in bioconjugation techniques, and we have the necessary equipment and resources to deliver high-quality AOCs tailored to meet the specific needs of our clients.

With our antibody-oligonucleotide conjugates Development services, we can help accelerate your research and development processes, providing you with the tools you need to take your projects to the next level. Whether you are looking to harness the power of AOCs in drug discovery, therapeutics, or diagnostics, we have the expertise to guide you through every step of the process.

Fig.1 Common Methods for the Synthesis and Labelling of Ab*-ssON Conjugates.^1,3

Services

AOCs are emerging as powerful tools for targeted therapy, diagnostics, and imaging of disease. By combining the specificity of antibodies with the versatility of oligonucleotides, Creative Biolabs offers a unique platform for precise AOC molecular recognition and manipulation.

1. Initial consultation: The client provides information on the target antibody and desired oligonucleotide sequence for conjugation.
2. Antibody and oligonucleotide preparation: The antibody and oligonucleotide are purified and characterized to ensure quality and stability before conjugation.
3. Conjugation reaction: The antibody and oligonucleotide are chemically conjugated using a suitable linker chemistry. Optimal conditions for conjugation, including pH, temperature, and reaction time, are determined through optimization experiments.
4. Conjugate purification: The antibody-oligonucleotide conjugate is purified to remove any unreacted components and impurities. Various purification methods, such as size exclusion chromatography or affinity chromatography, have been employed.
5. Characterization: The conjugate is extensively characterized to confirm the successful conjugation and assess its stability, binding affinity, and functionality. Techniques such as UV-Vis spectroscopy, mass spectrometry, and gel electrophoresis are used for characterization.
6. Bioconjugate validation: The antibody-oligonucleotide conjugate is tested in relevant biological assays to confirm its specificity, activity, and performance. Validation experiments may include ELISA, flow cytometry, or cell-based assays.
7. Documentation and reporting: A detailed report summarizing the development process, experimental results, and recommendations for further optimization is provided to the client.
8. Scale-up and production: Upon successful validation, the optimized conjugation process can be scaled up for larger production batches. The client may choose to proceed with manufacturing the antibody-oligonucleotide conjugate for research or commercial purposes.

Fig.2 Detection of HER2 Proteins Using an Antibody-DNA Conjugates.^2,3

Features

Our AOC services can \help you unlock the potential of antibody-oligonucleotide conjugates for your research or commercial applications. Key technical characteristics and advantages of our services include:

• High Specificity: Our AOC products have high specificity for their target antigens, allowing for precise targeting of oligonucleotides to specific cells or biomolecules.
• High Sensitivity: Our AOC products have a high affinity for their target antigens, leading to enhanced sensitivity of detection or therapeutic effects.
• Versatility: In our labs, various antibodies can be conjugated to a wide range of oligonucleotides, including siRNA, miRNA, antisense oligonucleotides, and aptamers, allowing for a variety of applications in research, diagnostics, and therapeutics.
• High Stability: Our products are generally stable in physiological conditions, ensuring long-lasting effects in vivo.
• Enhanced delivery: We can optimize the targeted delivery of AOCs to specific cells or tissues, improving the efficiency and efficacy of gene silencing or modulation.
• Multiplexing: Multiple oligonucleotides have been broadly used for antibody conjugation, allowing for simultaneous targeting of multiple genes or pathways.
• Reduced off-target effects: Our services can help reduce off-target effects of AOCs from different sources, minimizing unintended consequences in vivo.

Creative Biolabs is dedicated to antibody and oligonucleotide engineering, conjugation chemistry, and bioanalytical techniques. We can help you select the most suitable antibodies and oligonucleotides, optimize conjugation protocols, and validate the performance of the resulting conjugates in relevant biological assays. If you are interested in learning more about our antibody-oligonucleotide conjugates development services, please do not hesitate to contact us.

References

1. Lehot, Victor, et al. "Non-specific interactions of antibody-oligonucleotide conjugates with living cells." Scientific Reports 11.1 (2021): 5881.
2. Yan, Junhong, et al. "A universal approach to preparing reagents for DNA-assisted protein analysis." PLoS One 9.9 (2014): e108061.
3. under Open Access License CC BY 4.0, without modification.

For research use only. Not intended for any clinical use.