CytoStream™ Armenian Hamster Anti-IgG Isotype Control Recombinant Antibody (HMC-I1044)
CAT#: VS-0126-XU423
CytoStream™ Armenian Hamster Anti-IgG Isotype Control Recombinant Antibody (HMC-I1044) is a high-purity, non-reactive monoclonal reagent designed to serve as a negative control for hamster-derived primary antibodies. Produced through a recombinant platform, the clone ensures the absence of hybridoma-derived impurities and guarantees absolute lot-to-lot consistency. It is specifically validated for flow cytometry (FC) to help researchers confidently define gating boundaries and distinguish true antigen-specific signals from technical background in complex assays.
| Sub Cat | Product Name | Size | Formulation | Conjugate Group | Characteristics | Stability | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS-0126-XU423-1 | CytoStream™ Armenian Hamster Anti-IgG isotype Control Recombinant Antibody (HMC-I1044), APC-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | APC | Protein-based fluorochrome with excitation/emission at ~650/660 nm. Exhibits minimal spectral overlap with FITC and PE, ideal for multicolor panels. High brightness. | Sensitive to light and heat. Store at 4°C in the dark. Avoid repeated freeze-thaw cycles. Stable for ≥1 year under proper storage. | ||||||
| VS-0126-XU423-2 | CytoStream™ Armenian Hamster Anti-IgG isotype Control Recombinant Antibody (HMC-I1044), FITC-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | FITC | Small organic fluorophore (Ex/Em: 488/519 nm). High initial brightness but susceptible to photobleaching. Requires careful compensation with PE channel. | Sensitive to light and pH. Prone to rapid photobleaching during acquisition. Store at 4°C in the dark. Prepare working solutions fresh. | ||||||
| VS-0126-XU423-3 | CytoStream™ Armenian Hamster Anti-IgG isotype Control Recombinant Antibody (HMC-I1044), PE-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE | Phycobiliprotein fluorochrome (Ex/Em peak: 565/575 nm). One of the brightest fluorophores available, ideal for low-abundance antigen detection. Broad emission requires careful compensation. | Moderate stability. Sensitive to light, heat, and harsh fixatives. Store at 4°C in the dark. Avoid freeze-thaw cycles to maintain performance. | ||||||
| VS-0126-XU423-4 | CytoStream™ Purified Armenian Hamster Anti-IgG isotype Control Recombinant Antibody (HMC-I1044) | 100 ug | None | The native antibody without any fluorophore. Used as a primary antibody in indirect staining or for functional assays (blocking, neutralization). Requires a compatible fluorescent secondary antibody for detection. | Typically exhibits the highest intrinsic stability among all formats. Storage conditions are defined by the antibody's own formulation buffer. | |||||||
| VS-0126-XU423-5 | CytoStream™ Armenian Hamster Anti-IgG isotype Control Recombinant Antibody (HMC-I1044), PE-Cy5-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE-Cy5 | Tandem dye where PE transfers energy to Cy5 (Em ~667 nm). Critical: Use a Cy5 detector, not a PE filter. Prone to degradation of the tandem bond, causing uncoupled PE signal. | Low stability. Highly sensitive to fixation, light, and prolonged storage. Protect from light and analyze fixed samples promptly. | ||||||
| VS-0126-XU423-6 | CytoStream™ Armenian Hamster Anti-IgG isotype Control Recombinant Antibody (HMC-I1044), AF Dye (488nm)-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | AF Dye (488nm) | Synthetic green fluorogenic dye (Ex/Em ~490/520 nm). A photostable alternative to FITC with brighter signal and narrower emission spectrum, reducing spillover into PE channel. | Excellent photostability. Maintains signal integrity during prolonged acquisition. Tolerates fixation well and exhibits minimal pH sensitivity. | ||||||
| VS-0126-XU423-7 | CytoStream™ Armenian Hamster Anti-IgG isotype Control Recombinant Antibody (HMC-I1044), AF Dye (647nm)-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | AF Dye (647nm) | Synthetic far-red fluorogenic dye (Ex/Em ~650/665 nm). Spectrally similar to APC but with enhanced brightness and photostability. Ideal for replacing APC in demanding protocols. | Superior stability. Highly resistant to photobleaching and pH fluctuations. Compatible with harsh fixation and permeabilization protocols. | ||||||
| VS-0126-XU423-8 | CytoStream™ Armenian Hamster Anti-IgG isotype Control Recombinant Antibody (HMC-I1044), PE-Cy7-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE-Cy7 | Tandem dye (PE excited, Cy7 emits at ~785 nm). Critical for deep-red infrared detection. Essential: Use a Cy7 (780/60 nm) detector, not a PE filter. Susceptible to tandem bond degradation. | Low stability. Highly sensitive to fixation, light exposure, and storage time. Protect from light and analyze immediately post-fixation. | ||||||
| VS-0126-XU423-9 | CytoStream™ Armenian Hamster Anti-IgG isotype Control Recombinant Antibody (HMC-I1044), Percp-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | Percp | Protein-based fluorochrome (Ex/Em: 488/678 nm). Compatible with blue laser excitation, providing signal in red channel. Lower brightness compared to PE or APC but useful for spectral separation. | Moderate stability. PerCP is inherently photolabile and prone to degradation upon light exposure. Store strictly protected from light. | ||||||
| VS-0126-XU423-10 | CytoStream™ Armenian Hamster Anti-IgG isotype Control Recombinant Antibody (HMC-I1044), Pacific Blue-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | Pacific Blue | Synthetic blue fluorescent dye (Ex/Em: ~410/455 nm). Excellent for violet laser (405 nm) excitation. Low spectral overlap with green/red channel fluorophores, simplifying panel design. | High photostability. Tolerates common fixation protocols well. Store at 4°C in the dark for long-term stability. |
Specifications
- Host Species
- Armenian Hamster
- Type
- Monoclonal Antibody
- Specificity
- IgG isotype Control
- Applications
- FC
Product Property
- Clonality
- Monoclonal
- Storage
- Store at 4˚C.
- Shipping
- Ice Packs
Applications
- Application Notes
- Test: 5 μl/Test
Notice: This reagent is supplied in a pre-optimized, ready-to-use formulation for direct application at the specified volume per test in flow cytometry. The standard protocol is suitable for staining up to 1 million cells per assay. Optimal performance may require protocol adjustments based on specific experimental conditions.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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