CytoStream™ Mouse Anti-IgG1 Isotype Control Recombinant Antibody (MMC-I1042)
CAT#: VS-0126-XU390
CytoStream™ Mouse Anti-IgG1 Isotype Control Recombinant Antibody (MMC-I1042) is a premium, non-reactive reagent engineered to define negative populations in human and murine assays. This recombinant version of the classic clone ensures absolute lot-to-lot consistency and the absence of hybridoma-derived contaminants. Meticulously validated for flow cytometry (FC), it allows researchers to set confident quadrants and gates. Its structural uniformity provides a stable baseline for interpreting staining intensity in multi-color panels, making it a foundational component of experimental rigor.
Gene Expression
Normal Tissue
Figure 1 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_3.jpg
Normal Tissue
Figure 2 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_4.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_5_6.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (Karpas-707, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000211896-IGHG1
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| Sub Cat | Product Name | Size | Formulation | Conjugate Group | Characteristics | Stability | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS-0126-XU390-1 | CytoStream™ Mouse Anti-IgG1 isotype Control Recombinant Antibody (MMC-I1042), PE-Cy7-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE-Cy7 | Tandem dye (PE excited, Cy7 emits at ~785 nm). Critical for deep-red infrared detection. Essential: Use a Cy7 (780/60 nm) detector, not a PE filter. Susceptible to tandem bond degradation. | Low stability. Highly sensitive to fixation, light exposure, and storage time. Protect from light and analyze immediately post-fixation. | ||||||
| VS-0126-XU390-2 | CytoStream™ Mouse Anti-IgG1 isotype Control Recombinant Antibody (MMC-I1042), APC-Cy7-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | APC-Cy7 | Tandem dye (APC excited, Cy7 emits at ~785 nm). Provides a bright, far-red to infrared signal. Shares the APC-Cy7 detection channel with other Cy7 tandems. Also prone to bond degradation under stress. | Low stability. Similar sensitivity as PE-Cy7 to fixation and light. Requires strict adherence to dark, cold storage and prompt analysis. | ||||||
| VS-0126-XU390-3 | CytoStream™ Mouse Anti-IgG1 isotype Control Recombinant Antibody (MMC-I1042), FITC-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | FITC | Small organic fluorophore (Ex/Em: 488/519 nm). High initial brightness but susceptible to photobleaching. Requires careful compensation with PE channel. | Sensitive to light and pH. Prone to rapid photobleaching during acquisition. Store at 4°C in the dark. Prepare working solutions fresh. |
More Infomation
Specifications
- Host Species
- Mouse
- Type
- Monoclonal Antibody
- Specificity
- IgG1 isotype Control
- Applications
- FC
Product Property
- Clonality
- Monoclonal
- Storage
- Store at 4˚C.
- Shipping
- Ice Packs
Applications
- Application Notes
- Test: 5 μl/Test
Notice: This reagent is supplied in a pre-optimized, ready-to-use formulation for direct application at the specified volume per test in flow cytometry. The standard protocol is suitable for staining up to 1 million cells per assay. Optimal performance may require protocol adjustments based on specific experimental conditions.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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