dAbs scaffold protein anti-Hen egg lysozyme (HEL) (CAT#: SDS-L074)
The dAbs that bound hen egg lysozyme (HEL) was isolated from the library using Phage display techonology. After affinity maturation, dAbs variants were identified having affinities (KD=0.13 μM) for hen egg lysozyme (HEL) as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, the Golgi apparatus & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66182/1763_E3_3_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, the Golgi apparatus & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66182/1763_E3_3_selected.jpg

Figure 2 IHC staining of human salivary gland
(High expression. RNA expression: 21115.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_1_val_selected.jpg
(High expression. RNA expression: 21115.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_1_val_selected.jpg

Figure 3 IHC staining of human skeletal muscle
(Low expression. RNA expression: 34.0 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_6_val_selected.jpg
(Low expression. RNA expression: 34.0 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_6_val_selected.jpg

Figure 4 Colon
(Mucosal lymphoid cells
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_3.jpg
(Mucosal lymphoid cells
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_3.jpg

Figure 5 Kidney
(Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_9_5.jpg
(Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_9_5.jpg

Figure 6 Testis
(Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55/231_A_5_6.jpg
(Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55/231_A_5_6.jpg

Figure 7 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_8.jpg

Figure 8 RNA cell line category: Cell line enriched (OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000090382-LYZ
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000090382-LYZ
Specifications
- Scaffold Name
- dAbs
- Origin
- dAbs (human VH or VL single domain)
- Core Structure
- β-Sheet sandwich
- Variable Regions
- CDR 1, 2, 3
- Target
- Hen egg lysozyme (HEL)
- Species Reactivity
- Chicken
- Expression Host
- E. coli
- Affinity Constant
- 0.13 μM
- Applications
- ELISA; IHC; Microscopy; FC; WB; FuncS
Target
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Rabbit Monoclonal Antibody Products
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-3387z | Mouse Anti-LYZ Recombinant Antibody (clone 39H8) | WB, FC, ICC, IF, IHC | Mouse IgG2a |
PABZ-022 | Mouse Anti-LYZ Recombinant Antibody (clone D44.1) | WB, IF, FuncS | Mouse IgG |
PABW-022 | Human Anti-LYZ Recombinant Antibody (clone dAb HEL4) | ELISA | Human IgG |
PABC-023 | Recombinant Mouse Anti-Chicken LYZ Antibody (F10.6.6) | WB, IF, FuncS | IgG |
PABZ-023 | Mouse Anti-LYZ Recombinant Antibody (clone F9.13.7) | WB, IHC, IF, FuncS | Mouse IgG |
Customer Reviews and Q&As
Customer Review
Q&As
Exceptional Western Blot Clarity
Using this antibody in Western blotting has led to clear, distinct bands, which are crucial for our studies on protein expression. Its high specificity greatly reduces background noise, providing clearer results that bolster our experimental conclusions.
Consistent Results for ELISA
Our team has consistently achieved reproducible results using this antibody in ELISA assays. Its stability and high affinity for lysozyme provide accurate quantification, which is essential for our quantitative studies in immune function research.
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For Research Use Only. Not For Clinical Use.
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