Recently, the emergence of novel coronavirus (SARS-CoV-2) occurred in Wuhan, China in December 2019. This novel infectious disease now spreads around the world and more and more patients are diagnosed. In order to detect patients as soon as possible, therefore, the rapid and accurate detection tools of the novel coronavirus are becoming increasingly necessary and important. At Creative Biolabs, we mainly provide three kits for detecting SARS-CoV-2 infection, detection kits based on the fluorescent RT-PCR method, detection kits based on the colloidal gold method, and detection kits based on ELISA. It is noticed that their test results are for reference only and should not be the sole basis for diagnosis and treatment.Fig.1 The novel coronavirus (SARS-CoV-2).
This fluorescent RT-PCR detection kit (Detection for 3 Genes) is utilized for the in vitro qualitative detection of the ORF1ab gene, N gene and the E gene of SARS-CoV-2 in the upper respiratory tract specimens (nasopharyngeal and oropharyngeal extracts) and the lower respiratory tract specimens (bronchoalveolar lavage fluid (BALF) and deep cough sputum) from candidates via real-time PCR systems.
The kit based on the colloidal gold method is employed to qualitatively detect IgG/IgM antibodies of the novel coronavirus in human serum, plasma or whole blood in vitro. The principle of this detection kit is that IgM antibodies are made when individuals are first infected with new pathogens. When the body senses an invader, the level of IgM will rise and keep for a short time. However, IgG antibodies will then kick in and increase and keep for long-term protection. Checking whether IgM/IgG present in the samples by observing which lines are red on test cassette, lines indicates that candidates are infected or not.
The principle of this kit is based on the solid phase sandwich enzyme immunoassay technique. Firstly, a monoclonal antibody specific for SARS-CoV-2 Nucleoprotein (NP) has been pre-coated onto the well plate strips. Subsequently, samples are added to the plate. After incubating for a while, the plate will be washed and a horseradish peroxidase-conjugated anti-SARS-CoV-2 Nucleoprotein (NP) antibody will also be added. Finally, washing unbound antibody by the TMB substrate solution. If SARS-CoV-2 Nucleoprotein (NP) present in the sample, they will be bound by the immobilized antibody on the well plate. Moreover, there will be produced an antibody-antigen-antibody "sandwich complex" when added a horseradish peroxidase-conjugated anti-SARS-CoV-2 Nucleoprotein (NP) antibody. After washing unbound antibody, color develops in proportion to the amount of SARS-CoV-2 Nucleoprotein (NP) bound and will be observed by a microplate reader set to 450 nm.
These three detection kits based on different principles are the main diagnostic methods in the world nowadays. They easily perform or gets results fast. Moreover, they are more sensitive or/and accurate. Creative Biolabs offers detection kits for SARS-CoV-2 but also personalized experimental design and excellent support based on customers’ special requirements. Except that, we also provide SARS-CoV-2 antibodies for scientific researches. If you want to know more information about detection kits and antibodies of SARS-CoV-2, please don’t hesitate to contact us.
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