High-Performance Elispot Kits for Sensitive Immune Monitoring

Understanding immune responses at the cell level is critical for advancing research in immunology, infectious diseases, oncology, and vaccine development. Our Elispot (Enzyme-Linked ImmunoSpot) Kits are designed to provide sensitive, specific, and reproducible detection of cytokine-secreting cells, helping researchers make more informed decisions faster. With decades of assay development expertise, optimized components, and comprehensive technical support, our Elispot Kits are trusted by leading research institutions.

What is Elispot?

Elispot is a plate-based immunoassay that enables the detection and enumeration of cytokine- or antibody-secreting cells at the cell level. Unlike bulk assays that average out signals across populations, Elispot highlights the functional heterogeneity of immune cells, offering more accurate and meaningful insights.

It is particularly useful for:

• Detecting antigen-specific T-cell responses
• Quantifying cytokine-producing cells in low-frequency populations
• Evaluating vaccine-induced immunity
• Studying immune dysregulation in disease models

Principle of the Elispot Assay

The assay is based on a sandwich immunoassay principle, adapted to a membrane surface that captures secreted proteins directly underneath the secreting cells.

Workflow Overview:

1. Plate Coating

Plates are pre-coated with monoclonal capture antibodies specific to the target cytokine (e.g., IFN-γ, IL-2).

2. Sample Incubation

Freshly isolated immune cells (PBMCs, splenocytes, etc.) are stimulated with antigens or mitogens. Activated cells secrete cytokines that are immediately captured by the coated antibodies.

3. Detection Step

After washing, a biotinylated detection antibody binds to the captured cytokine. This is followed by an enzyme (e.g., streptavidin-ALP or HRP) that catalyzes a colorimetric reaction.

4. Spot Development

Upon substrate addition, dark-colored spots form at the sites of secretion, each representing a single cytokine-producing cell.

5. Analysis

Spots are counted manually or using automated Elispot readers. The number of spots correlates directly with the frequency of antigen-specific cells in the sample.

Key Features & Benefits

• Exceptional Sensitivity

Detects rare cytokine-secreting cells, even at frequencies as low as 1 in 300,000. Ideal for applications involving low-frequency antigen-specific T cells, such as tumor-infiltrating lymphocytes or vaccine responders.

• Low Background, High Signal-to-Noise Ratio

Our optimized blocking buffers and wash steps minimize background staining and non-specific signals, ensuring clear, countable spots.

• Flexible Formats

Available as:

Pre-coated kits for convenience and time savings

Uncoated kits for researchers who prefer to coat their own antibodies or need assay flexibility

• Multiplexing Options

Dual-color Elispot kits allow the simultaneous detection of two cytokines (e.g., IFN-γ + IL-2), enhancing data output per sample and revealing functional cell subsets.

• Validated for Multiple Species

Kits are available for human, mouse, rat, non-human primates, and other model organisms.

Applications Across Immunology and Beyond

Our Elispot kits are widely used in both basic and applied research settings, including:

• Vaccine Development

Quantify antigen-specific cellular immunity following vaccination or viral challenge. Evaluate T-helper (Th1/Th2/Th17) and cytotoxic T lymphocyte responses.

• Cancer Immunology

Assess tumor-specific immune responses and identify T-cell activation in response to neoantigens or immune checkpoint therapies.

• Autoimmunity & Allergies

Monitor dysregulated cytokine production in autoimmune conditions (e.g., rheumatoid arthritis, lupus) or in response to allergens.

• Transplantation Research

Investigate donor-reactive T-cell frequencies and immune tolerance mechanisms.

• Infectious Disease Studies

Study cellular immunity against pathogens such as HIV, HBV, SARS-CoV-2, TB, or malaria.

Why Partner with Us?

When you choose our Elispot kits, you're choosing:

• Proven Quality
• Fast Lead Times
• Experienced Support
• Publication-Ready Results

Contact Us Today

Whether you're conducting a vaccine trial, monitoring tumor-specific responses, or exploring novel immunotherapies, our Elispot kits offer the precision and reliability your research demands. Let's work together to advance immune monitoring - one spot at a time.

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For Research Use Only. Not For Clinical Use.