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FAK-Src Signaling

Representative FAK-Src Signaling Full List of Targets in FAK-Src Signaling Tested Data-Supported Products for Targeting FAK-Src Signaling

Focal Adhesion Kinase (FAK) and Src are two non-receptor tyrosine kinases in the realm of cellular signaling, playing critical roles in various biological processes that govern cell behavior. FAK-Src signaling pathway, a central conduit of transducing extracellular signals into intracellular actions, orchestrates a wide array of cellular functions including adhesion, migration, proliferation, survival, and differentiation. Focal Adhesion Kinase (FAK) and Src are non-receptor tyrosine kinases that, upon activation, form a complex initiating a cascade of signaling events crucial for the dynamic regulation of focal adhesions—specialized sites where cells adhere to the extracellular matrix. The interaction between FAK and Src is not merely structural; it is a functional synergy that amplifies signaling pathways, influencing cell morphology and motility, which are essential for tissue development, wound healing, and immune responses. Moreover, the FAK-Src axis is critically involved in the pathological context, contributing to the progression of various diseases, including cancer, where it promotes tumor growth, angiogenesis, and metastasis. By phosphorylating downstream targets, this signaling duo modulates the actin cytoskeleton, leading to changes in cell shape and movement. Furthermore, their role in the regulation of gene expression and cell cycle progression underscores their importance in cellular homeostasis and disease.

Figure 1 Model of FAK signaling upon activation by integrins, growth factor receptors, and/or cytokine receptors. (Chen, 2016) Figure 1 Model of FAK signaling upon activation by integrins, growth factor receptors, and/or cytokine receptors. (Chen, 2016)

Representative Targets in FAK-Src Signaling

PTK2

PTK2, also known as Focal Adhesion Kinase (FAK), is a non-receptor tyrosine kinase that plays a pivotal role in signaling pathways associated with cell migration, proliferation, and survival. It is predominantly located at focal adhesions, which are specialized sites where cells adhere to the extracellular matrix (ECM), and serves as a key mediator in transducing signals from integrins and growth factor receptors to the interior of the cell. This kinase is activated by autophosphorylation at tyrosine 397, which then becomes a binding site for Src family kinases, further propagating signaling cascades that influence gene expression, cell cycle progression, and apoptosis. PTK2's role extends to the regulation of cellular architecture and the dynamics of the actin cytoskeleton, making it instrumental in the process of cell motility and the metastatic spread of cancer cells. Its expression and activity are tightly regulated, and dysregulation has been linked to a variety of diseases, including cancer, where it is often overexpressed and associated with increased invasive and metastatic potential.

Recommended Mouse Anti-PTK2 mAb (CAT#: ZG-0331J)
Figure 2 Mouse Anti-PTK2 Recombinant Antibody (ZG-0331J) in IHC
Figure 2 Mouse Anti-PTK2 Recombinant Antibody (ZG-0331J) in IHC. Immunohistochemistry analysis of paraffin-embedded cervices tumor with DAB staining using FAK Monoclonal Antibody.
Recommended Rabbit Anti-PTK2 mAb (CAT#: ZG-0332J)
Figure 3 Mouse Anti-PTK2 Recombinant Antibody (ZG-0332J) in IF
Figure 3 Mouse Anti-PTK2 Recombinant Antibody (ZG-0332J) in IF. Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20™and using FAK mouse mAb (dilution 1:200).

Src

Src is a proto-oncogene that encodes a tyrosine kinase protein, and is recognized as a critical component of multiple signaling pathways in both normal and cancerous cells. This kinase functions by phosphorylating tyrosine residues on target proteins, thereby altering their activity and initiating a cascade of downstream effects that influence cell behavior. In normal physiological conditions, Src activity is tightly regulated through phosphorylation at its C-terminal tyrosine residue, which maintains it in an inactive conformation. However, in pathological states such as cancer, Src is often found to be overexpressed or hyperactivated, leading to aberrant cellular proliferation, angiogenesis, and metastasis. Beyond oncogenesis, Src plays significant roles in bone metabolism, where it is involved in osteoclast function and bone resorption. Thus, Src represents a critical node in the complex web of cellular signaling, with its dysregulation implicated in a range of diseases, making it a target of interest for therapeutic intervention.

Recommended Rabbit Anti-Src mAb (CAT#: ZG-0577U)
Figure 4 Rabbit Anti-SRC Antibody (ZG-0577U) in IP
Figure 4 Rabbit Anti-SRC Antibody (ZG-0577U) in IP. Overlay histogram showing SH-SY5Y cells stained with ZG-0577U (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10, 000 events was performed.

Full List of Targets in FAK-Src Signaling

Biomarker Alternative Names Gene ID UniProt ID Roles
PTK2 PTK2; FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK 5747 Q05397 PTK2 protein tyrosine kinase 2 (PTK2), also known as focal adhesion kinase (FAK), is a protein that, in humans, is encoded by the PTK2 gene.
SRC SRC Proto-Oncogene, Non-Receptor Tyrosine Kinase; V-Src Avian Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog; Proto-Oncogene C-Src; EC 2.7.10.2; P60-Src; SRC1; Proto-Oncogene Tyrosine-Protein Kinase Src; Protooncogene SRC, Rous Sarcoma 396442 P00523 This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Tested Data-Supported Products for Targeting FAK-Src Signaling

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Reference
  1. Chen, H., Cheng, C.Y. (2016). Focal Adhesion Kinase (FAK). In: Choi, S. (eds) Encyclopedia of Signaling Molecules. Springer, New York, NY.

For research use only. Not intended for any clinical use.

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