Overview

As a crucial role in the human immune system, antibodies have become invaluable tools in both research and clinical settings. In research, they are widely used in techniques such as IHC, ELISA, WB, etc. For therapeutics, monoclonal antibodies have transformed the landscape of medicine, offering targeted and effective treatments for a wide range of diseases, such as cancer, autoimmune disorders, and infectious diseases. With over 30 FDA-approved monoclonal antibody therapies currently available, the demand for high-quality, safe, and stable antibody production continues to grow.

Fig.1 Various applications mediated by monoclonal antibodies.¹

High Stable Antibody Production and QC Service at Creative Biolabs

Creative Biolabs operates in the efficient and accurate development of stable cell lines capable of constitutively expressing high-quality monoclonal antibodies. Upon receiving the sequence or DNA vector, we perform codon optimization to improve the expression rate of antibodies. The interested gene is cloned into the appropriate expression plasmid through gene synthesis and plasmid construction. Subsequently, we employ the most suitable method to transfect the expression plasmid into the customer-selected expression system to establish stable cell lines for subsequent stable antibody expression.

After culturing and harvesting, we collect the target protein for subsequent purification processes to obtain high-purity, low-potential-hazard antibodies. Undergoes strict quality control, we deliver the antibody products that meet the requirements, the QC results, and the DNA construct (upon request) to customers. Throughout our service, we provide multiple types of expression cell lines such as HEK293 and CHO cells for customers to construct desired stable antibody production cell lines. In addition, we also offer optional transfection approaches and purification technologies to achieve efficient antibody expression and high-purity antibody collection. Backed up by our robust antibody production platform and experienced research team, we enable the flexible (milligram to gram) scale of high-quality antibody delivery for global customers in the desired timelines.

Fig.2 Our service process.

Producible Antibody Formats at Creative Biolabs

Our stable antibody expression and purification service is suitable for various antibody formats, which include:

• Full-Length Antibodies (IgG);
• Antibody Fragments: Fab Fragment, scFv Fragment, Fc Fusion Proteins;
• Bispecific Antibodies.

Available Expression Cell Line

The choice of expression host cell line is crucial for successful stable antibody production. Here we provide several commonly used expression cell lines:

• Mammalian Cell Lines: CHO cells, HEK293 cells;
• Other Cell Lines: Insect cell lines; Yeast, etc.

Selective Transfection Approaches

Transfection is a crucial step in introducing foreign DNA into host cells to establish stable antibody-producing cell lines. Here are several commonly used technologies:

• Calcium Phosphate Transfection: A classic method involving the formation of DNA-calcium phosphate precipitates, which are taken up by cells.
• Lipofection: Lipid-based transfection reagents form complexes with DNA, facilitating cellular uptake.
• Electroporation: Electroporation is a physical method that utilizes electrical pulses to transiently permeabilize cell membranes, enabling the introduction of exogenous DNA or other molecules.
• Viral Transduction: Viral vectors, such as lentiviruses or retroviruses, are used to integrate the gene of interest into the host cell genome.

Advanced Purification Techniques

Depending on the antibody format and desired purity level, we customize the appropriate purification technologies to achieve maximum antibody production. Here are several commonly used methods:

• Protein A/G Affinity Chromatography
• Immobilized Metal Affinity Chromatography (IMAC)

Attractive Features

• Various cell lines that are exceptional in quality, stability, and productivity
• Rapid screening of stable cell clones
• Consistent and long-term production
• Reduced production costs
• Strict quality control
• Customized services
• Rapid timeline (~three months)

Work with Creative Biolabs

As a leading company in antibody production, Creative Biolabs is constantly improving antibody production methods and conditions to broaden production advantages, enabling high-quality antibody products to be delivered timely for each customer. If you are looking for a smart stable antibody expression and purification supplier, please feel free to contact us.

For research use only. Not intended for any clinical use.